| CPC G01N 33/6851 (2013.01) [B01L 3/502715 (2013.01); B01L 3/50273 (2013.01); G01N 1/40 (2013.01); G01N 30/00 (2013.01); G01N 33/6848 (2013.01); H01J 49/0404 (2013.01); H01J 49/165 (2013.01); B01L 2300/0645 (2013.01); B01L 2300/0654 (2013.01); B01L 2300/0816 (2013.01); B01L 2300/0887 (2013.01); B01L 2400/0406 (2013.01); B01L 2400/0421 (2013.01); G01N 2001/4038 (2013.01)] | 20 Claims |

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1. A system comprising:
a microfluidic device comprising:
a separation channel for electrophoretic separation of an analyte mixture, the separation channel comprising a window, a proximal end, and a distal end;
a mobilization channel fluidly coupled to the separation channel, the mobilization channel for delivering a mobilization electrolyte to the separation channel for mobilization of separated analytes;
a first electrode electrically coupled with the proximal end of the separation channel, wherein the proximal end of the separation channel is in fluid communication with an inlet of the microfluidic device;
a second electrode electrically coupled with the distal end of the separation channel, wherein the distal end of the separation channel is opposite the proximal end of the separation channel, and
a third electrode electrically coupled with the mobilization channel; and
an imaging unit disposed to capture light transmitted through the window of the separation channel.
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