US 12,326,447 B2
Lateral flow assay for detecting multiple proteins of a pathogen
Mehdi Hatamian, Mission Viejo, CA (US)
Assigned to 2Pi-Sigma Corp., Newport Beach, CA (US)
Filed by 2Pi-Sigma Corp., Newport Beach, CA (US)
Filed on Oct. 4, 2022, as Appl. No. 17/959,309.
Application 17/959,309 is a continuation of application No. 17/232,553, filed on Apr. 16, 2021, granted, now 11,493,509.
Claims priority of provisional application 63/011,282, filed on Apr. 16, 2020.
Prior Publication US 2023/0026892 A1, Jan. 26, 2023
Int. Cl. G01N 33/543 (2006.01)
CPC G01N 33/54388 (2021.08) [G01N 2469/20 (2013.01)] 17 Claims
OG exemplary drawing
 
1. A lateral flow assay device, comprising:
a test strip configured to receive a sample fluid and detect a presence of antibodies to a target pathogen, each antibody specific to one of a plurality of proteins of the target pathogen, each antibody belonging to one of a plurality of classes of antibodies, the test strip comprising:
a conjugate pad,
wherein the conjugate pad is configured to receive a quantity of fluid after a start of a test and move the fluid by capillary action, and
wherein the conjugate pad is configured to contain a plurality of classes of antibodies to the target pathogen that are conjugated with a label, and
a membrane fluidly connected to the conjugate pad,
wherein the membrane is configured to move the fluid by capillary action, and
wherein the membrane comprises a test line comprising a plurality of different proteins of the target pathogen immobilized on the test line.