US 12,326,446 B2
Sequential lateral flow device
Gregory M. Lawrence, Marlborough, MA (US); Adam P. Lousararian, Marlborough, MA (US); Yli Remo Vallejo, Marlborough, MA (US); and Lisa Shinefeld, Marlborough, MA (US)
Assigned to Verax Biomedical Incorporated, Marlborough, MA (US)
Filed by Verax Biomedical Incorporated, Marlborough, MA (US)
Filed on Apr. 4, 2022, as Appl. No. 17/713,181.
Application 17/713,181 is a division of application No. 16/047,980, filed on Jul. 27, 2018, granted, now 11,293,922, issued on Apr. 5, 2022.
Claims priority of provisional application 62/537,701, filed on Jul. 27, 2017.
Prior Publication US 2022/0236265 A1, Jul. 28, 2022
Int. Cl. G01N 33/543 (2006.01); B01L 3/00 (2006.01); G01N 33/53 (2006.01); G01N 33/566 (2006.01); G01N 33/569 (2006.01); G01N 33/577 (2006.01)
CPC G01N 33/54388 (2021.08) [B01L 3/5023 (2013.01); B01L 3/502715 (2013.01); G01N 33/5304 (2013.01); G01N 33/566 (2013.01); G01N 33/56911 (2013.01); G01N 33/577 (2013.01); B01L 2300/069 (2013.01); B01L 2300/0825 (2013.01); B01L 2400/0406 (2013.01)] 18 Claims
OG exemplary drawing
 
1. A lateral flow device for detecting a plurality of target analytes in a liquid sample, the device comprising:
a housing unit comprising an inner surface that defines a cavity in the housing unit;
a capillary flow bed residing in the cavity, wherein the capillary flow bed transports the liquid sample from a proximal region of the capillary flow bed to a distal region of the capillary flow bed;
a buffer-receiving zone comprising a buffer-receiving pad (PB) and a conjugate pad (PC), the conjugate pad comprising a signaling agent for providing a detectable signal;
a capture zone (C) comprising a plurality of immobilized capture agents;
a sample-receiving zone disposed between the buffer-receiving zone and the capture zone, the sample-receiving zone comprising a sample-receiving pad (PS), a transfer pad (PT), and a substantially impermeable backing disposed between the sample-receiving zone and the capillary flow bed and extending at least partially underneath the sample-receiving pad and the transfer pad, the transfer pad comprising a plurality of binding agents that specifically bind to a plurality target analytes;
a bibulous reservoir pad (R) disposed in the distal region of the capillary flow bed;
a first inlet (I1) in said housing for introducing the liquid sample into the sample-receiving pad, wherein the impermeable backing inhibits contact between the liquid sample and the capillary flow bed in the sample-receiving zone, thereby reducing backflow of the sample in a proximal direction;
a second inlet (I2) in said housing for introducing a buffer into the buffer-receiving pad, wherein the buffer mobilizes the signaling agent to obtain a mobilized signaling agent; and
a reading window defined in the housing unit over the capture zone for observing a detectable signal produced by an interaction of the binding agent and the signaling agent in the presence of the target analyte;
wherein the bibulous reservoir pad draws the liquid sample and the mobilized signaling agent in the distal flow direction, and wherein the liquid sample flows along the capillary flow bed into the capture zone before the mobilized signaling agent flows into the capture zone.