US 12,326,444 B2
Development and parameter assessment for vertically aligned platinum wire aptasensor arrays for impedimetric detection of cardiac biomarkers
Prashant N. Kumta, Pittsburgh, PA (US); and Mitali Patil, Pittsburgh, PA (US)
Assigned to UNIVERSITY OF PITTSBURGH-OF THE COMMONWEALTH SYSTEM OF HIGHER EDUCATION, Pittsburgh, PA (US)
Filed by UNIVERSITY OF PITTSBURGH—OF THE COMMONWEALTH SYSTEM OF HIGHER EDUCATION, Pittsburgh, PA (US)
Filed on Sep. 10, 2021, as Appl. No. 17/471,788.
Application 17/471,788 is a continuation of application No. 15/765,306, granted, now 11,156,609, previously published as PCT/US2016/055299, filed on Oct. 4, 2016.
Claims priority of provisional application 62/237,104, filed on Oct. 5, 2015.
Prior Publication US 2021/0405038 A1, Dec. 30, 2021
This patent is subject to a terminal disclaimer.
Int. Cl. G01N 33/543 (2006.01); G01N 27/327 (2006.01); G01N 33/68 (2006.01)
CPC G01N 33/5438 (2013.01) [G01N 33/6887 (2013.01); G01N 27/327 (2013.01); G01N 2800/32 (2013.01)] 16 Claims
OG exemplary drawing
 
1. A portable, handheld device to detect a cardiac biomarker of interest in a patient, comprising:
at least one aptasensor, comprising:
vertically aligned platinum wires cast in an epoxy substrate;
a biological sensor agent comprising an immobilization agent, applied to the vertically aligned platinum wires; and
one or more aptamers selected to interact with the immobilization agent and bind with the cardiac biomarker of interest;
a test strip substrate comprising the at least one aptasensor;
a bodily fluid sample derived from the patient in contact with the test strip substrate; and
a displayed result comprising a visual change present on a surface of the test strip substrate or a visual change absent on a surface of the test strip substrate,
wherein the device is configured such that in its use, presence of the visual change on the surface of the test strip substrate is indicative of a presence of the cardiac biomarker of interest in the bodily fluid sample and binding with the one or more aptamers, and absence of the visual change on the surface of the test strip substrate is indicative of an absence of the cardiac biomarker of interest in the bodily fluid sample and binding with the one or more aptamers; and
a pre-calibrated chart that shows one or more visual changes for correlation with the displayed result.