US 12,325,884 B2
Riboswitch-based fluoride sensing in cell-free extract
Julius B. Lucks, Evanston, IL (US); Walter M. Thavarajah, Chicago, IL (US); Adam D. Silverman, Chicago, IL (US); and Michael C. Jewett, Evanston, IL (US)
Assigned to Northwestern University, Evanston, IL (US)
Appl. No. 17/593,026
Filed by Northwestern University, Evanston, IL (US)
PCT Filed Mar. 3, 2020, PCT No. PCT/US2020/020800
§ 371(c)(1), (2) Date Sep. 3, 2021,
PCT Pub. No. WO2020/185451, PCT Pub. Date Sep. 17, 2020.
Claims priority of provisional application 62/813,368, filed on Mar. 4, 2019.
Prior Publication US 2022/0170117 A1, Jun. 2, 2022
Int. Cl. G01N 33/84 (2006.01); C12Q 1/6897 (2018.01); G01N 33/58 (2006.01)
CPC C12Q 1/6897 (2013.01) [G01N 33/58 (2013.01); G01N 33/84 (2013.01); C12N 2310/16 (2013.01)] 17 Claims
OG exemplary drawing
 
1. A method of detecting fluoride in a liquid sample, comprising:
(i) obtaining a sample which may or may not contain fluoride;
(ii) adding the sample to a cell-free protein synthesis (CFPS) reaction comprising a fluoride-sensing riboswitch, wherein if fluoride is present in the sample at a concentration of about 0.05 mM or higher, then a detectable output is generated within less than about 300 minutes.