	US 12,325,869 B2
	Oligosaccharide debranching enzyme mutant and use thereof in glucose mother liquor
Zhaofeng Li, Wuxi (CN); Shixia Xi, Wuxi (CN); Zhengbiao Gu, Wuxi (CN); Caiming Li, Wuxi (CN); Xiao Zhang, Wuxi (CN); Zexi Li, Wuxi (CN); and Xiaofeng Ban, Wuxi (CN)
Assigned to JIANGNAN UNIVERSITY, Wuxi (CN)
Filed by JIANGNAN UNIVERSITY, Wuxi (CN)
Filed on Apr. 12, 2024, as Appl. No. 18/634,776.
Application 18/634,776 is a continuation of application No. PCT/CN2023/103662, filed on Jun. 29, 2023.
Claims priority of application No. 202211504329.2 (CN), filed on Nov. 28, 2022.
Prior Publication US 2025/0122484 A1, Apr. 17, 2025
Int. Cl. C12N 15/67 (2006.01); C12N 9/10 (2006.01); C12N 9/24 (2006.01); C12N 15/70 (2006.01); C12P 19/02 (2006.01)

CPC C12N 9/1055 (2013.01) [C12P 19/02 (2013.01); C12Y 204/0101 (2013.01)]

10 Claims

1. An oligosaccharide debranching enzyme mutant, obtained by mutating valine at position 219 in an amino acid sequence as shown in SEQ ID NO: 1 into alanine.