	US 12,325,867 B2
	Organoid produced using carrier for cell culture, and method for evaluating drug toxicity using same
Sun Woong Kang, Daejeon (KR); and Hye Eun Shim, Daejeon (KR)
Assigned to Korea Research Institute of Chemical Technology, Daejeon (KR)
Appl. No. 17/283,516
Filed by Korea Research Institute of Chemical Technology, Daejeon (KR)
PCT Filed Nov. 12, 2019, PCT No. PCT/KR2019/095042 § 371(c)(1), (2) Date Apr. 7, 2021, PCT Pub. No. WO2020/101461, PCT Pub. Date May 22, 2020.
Claims priority of application No. 10-2018-0139220 (KR), filed on Nov. 13, 2018.
Prior Publication US 2021/0388313 A1, Dec. 16, 2021
This patent is subject to a terminal disclaimer.
Int. Cl. C12N 5/00 (2006.01); G01N 33/50 (2006.01)

CPC C12N 5/0075 (2013.01) [G01N 33/5014 (2013.01); C12N 2500/76 (2013.01); C12N 2531/00 (2013.01); C12N 2533/54 (2013.01); C12N 2533/80 (2013.01)]

7 Claims

1. A method for organoid production, comprising co-culturing mammalian cells and a carrier for three-dimensional (3D) mammalian cell culture, the carrier comprising microcapsules each having a wall containing gelatin and a natural polymer, surrounding a core containing an oil, and dextrin palmitate as an oil thickener, to produce organoids, wherein

the natural polymer is one or more selected from the group consisting of Arabic gum, hyaluronic acid, guar gum, pectin, xanthan gum, locust bean gum, tamarind gum, tragacanth gum, gum ghatti, locust bean gum, Konjac gum, agar, Carragheenan, furcellaran, and gellan, and wherein

the oil is one or more selected from the group consisting of olive oil, camellia oil, castor oil, palm oil, Jojoba oil, almond oil, grapeseed oil, herbal oil, rose oil, coconut oil, moringa oil, rice bran oil, apricot kernel oil, sunflower oil, meadowfoam seed oil, Abyssinian oil, and squalane.