	US 12,325,856 B2
	Recombinant virus products and methods for inhibition of expression of myotilin
Scott Quenton Harper, Powell, OH (US); and Jian Liu, Savoy, IL (US)
Assigned to RESEARCH INSTITUTE AT NATIONWIDE CHILDREN'S HOSPITAL, Columbus, OH (US)
Filed by NATIONWIDE CHILDREN'S HOSPITAL, INC., Columbus, OH (US)
Filed on Jul. 13, 2021, as Appl. No. 17/374,147.
Application 17/374,147 is a division of application No. 16/144,432, filed on Sep. 27, 2018, granted, now 11,091,760.
Application 16/144,432 is a continuation of application No. 14/853,517, filed on Sep. 14, 2015, granted, now 10,196,636.
Application 14/853,517 is a continuation in part of application No. 14/058,909, filed on Oct. 21, 2013, granted, now 9,133,482.
Application 14/058,909 is a continuation in part of application No. PCT/US2012/034408, filed on Apr. 20, 2012.
Claims priority of provisional application 61/478,012, filed on Apr. 21, 2011.
Prior Publication US 2022/0064641 A1, Mar. 3, 2022
Int. Cl. C12N 15/86 (2006.01); C07K 14/47 (2006.01); C12N 15/113 (2010.01)

CPC C12N 15/113 (2013.01) [C07K 14/4707 (2013.01); C12N 15/1138 (2013.01); C12N 15/86 (2013.01); C12N 2310/14 (2013.01); C12N 2310/141 (2013.01); C12N 2320/32 (2013.01); C12N 2330/51 (2013.01); C12N 2750/14143 (2013.01)]

10 Claims

1. A recombinant adeno-associated virus encoding a myotilin (MYOT) microRNA (miRNA) comprising an antisense guide strand as set out in any one of SEQ ID NOs: 7701, 7798, 7945, 7967, 7990, 8057, 8078, and 8148, wherein the recombinant adeno-associated virus lacks rep and cap genes.