US 12,325,846 B2
Open-top microfluidic devices and methods for simulating a function of a tissue
Antonio Varone, Newton, MA (US); Norman Wen, Newton, MA (US); Daniel Levner, Brookline, MA (US); Richard Novak, Jamaica Plain, MA (US); Lori McPartlin, North Billerica, MA (US); Donald E. Ingber, Boston, MA (US); Youngjae Choe, Somerville, MA (US); Lian Leng, LaSalle (CA); and Justin K. Nguyen, Medford, MA (US)
Assigned to President and Fellows of Harvard College, Cambridge, MA (US)
Filed by President and Fellows of Harvard College, Cambridge, MA (US)
Filed on Mar. 13, 2024, as Appl. No. 18/604,469.
Application 18/604,469 is a continuation of application No. 17/820,174, filed on Aug. 16, 2022, abandoned.
Application 17/820,174 is a continuation of application No. 15/781,078, granted, now 11,505,773, issued on Nov. 22, 2022, previously published as PCT/US2016/064798, filed on Dec. 2, 2016.
Claims priority of provisional application 62/263,225, filed on Dec. 4, 2015.
Prior Publication US 2024/0218309 A1, Jul. 4, 2024
Int. Cl. C12M 3/00 (2006.01); B01L 3/00 (2006.01); C12M 1/00 (2006.01); C12M 1/12 (2006.01); C12M 3/06 (2006.01); C12N 5/071 (2010.01); C12N 5/077 (2010.01); C12M 1/34 (2006.01); C12M 3/04 (2006.01)
CPC C12M 23/16 (2013.01) [C12M 21/08 (2013.01); C12M 25/02 (2013.01); C12M 25/14 (2013.01); C12N 5/0629 (2013.01); C12N 5/0656 (2013.01); C12N 5/0679 (2013.01); C12N 5/069 (2013.01); B01L 3/5027 (2013.01); B01L 2300/069 (2013.01); C12M 3/04 (2013.01); C12M 29/10 (2013.01); C12M 41/46 (2013.01)] 7 Claims
OG exemplary drawing
 
1. A method of seeding cells, comprising:
(a) providing a microfluidic device comprising a first chamber, the first chamber including a three-dimensional gel matrix disposed therein, and a second chamber, said second chamber separated from the first chamber by a membrane, the gel matrix positioned under a removable cover that comprises a fluidic chamber, wherein said fluidic chamber of said removable cover substantially aligns with all of an open top region of the first chamber;
(b) removing the removable cover thereby exposing the gel matrix; and
(c) seeding cells within the gel matrix.