	US 12,325,736 B2
	Transduced T cells expressing human SSTR2 and application thereof
Moonsoo Jin, New York, NY (US)
Assigned to Cornell University, Ithaca, NY (US)
Filed by Cornell University, Ithaca, NY (US)
Filed on Mar. 21, 2023, as Appl. No. 18/187,268.
Application 18/187,268 is a division of application No. 16/787,539, filed on Feb. 11, 2020, granted, now 11,634,471.
Application 16/787,539 is a division of application No. 15/675,419, filed on Aug. 11, 2017, granted, now 10,577,408, issued on Mar. 3, 2020.
Claims priority of provisional application 62/419,817, filed on Nov. 9, 2016.
Claims priority of provisional application 62/383,139, filed on Sep. 2, 2016.
Prior Publication US 2023/0265165 A1, Aug. 24, 2023
This patent is subject to a terminal disclaimer.
Int. Cl. C07K 14/72 (2006.01); A61K 38/17 (2006.01); A61K 40/11 (2025.01); A61K 40/31 (2025.01); A61K 40/42 (2025.01); A61K 51/08 (2006.01); C07K 14/705 (2006.01); C07K 14/725 (2006.01); C12N 5/0783 (2010.01); A61K 39/00 (2006.01)

CPC C07K 14/723 (2013.01) [A61K 38/1796 (2013.01); A61K 40/11 (2025.01); A61K 40/31 (2025.01); A61K 40/421 (2025.01); A61K 51/083 (2013.01); A61K 51/088 (2013.01); C07K 14/7051 (2013.01); C07K 14/70521 (2013.01); C07K 14/70553 (2013.01); C12N 5/0636 (2013.01); A61K 2039/515 (2013.01); A61K 2039/54 (2013.01); A61K 2239/31 (2023.05); A61K 2239/38 (2023.05); C07K 2319/03 (2013.01); C07K 2319/74 (2013.01); C12N 2510/00 (2013.01)]

14 Claims

1. A method for monitoring T cell distribution in a patient, comprising the steps of:

incubating transduced T cells with a radioactive label that binds to SSTR2,

intravenously infusing the labelled T cells into a patient, and

detecting the labelled T cell distribution by PET/CT imaging;

wherein the transduced T cells express SSTR2 and chimeric antigen receptor (CAR) specific to ICAM-1, wherein the CAR comprises an I domain of the αL subunit of human lymphocyte function-associated antigen-1, and the I domain is a mutant of the wild-type I domain comprising amino acids 130-310 of SEQ ID NO: 1, with a mutation of F292A, F292S, L289G, F292G, or F265S.