US 12,325,730 B2
Compositions and methods for producing high secreted yields of recombinant proteins
Joshua Kittleson, Pleasant Hill, CA (US); Thomas Stevens, San Francisco, CA (US); Rena Hill, Oakland, CA (US); Carlos Gustavo Pesce, San Francisco, CA (US); David N. Breslauer, San Francisco, CA (US); and Daniel M. Widmaier, San Francisco, CA (US)
Assigned to BOLT THREADS, INC., Emeryville, CA (US)
Filed by BOLT THREADS, INC., Emeryville, CA (US)
Filed on Jun. 26, 2023, as Appl. No. 18/341,669.
Application 18/341,669 is a continuation of application No. 17/695,219, filed on Mar. 15, 2022, granted, now 11,725,030.
Application 17/695,219 is a continuation of application No. 15/920,291, filed on Mar. 13, 2018, granted, now 11,306,127, issued on Apr. 19, 2022.
Application 15/920,291 is a continuation of application No. PCT/US2018/021812, filed on Mar. 9, 2018.
Claims priority of provisional application 62/470,144, filed on Mar. 10, 2017.
Prior Publication US 2024/0150416 A1, May 9, 2024
Int. Cl. C07K 14/435 (2006.01); C07K 14/395 (2006.01); C12P 21/02 (2006.01)
CPC C07K 14/43586 (2013.01) [C07K 14/395 (2013.01); C12P 21/02 (2013.01); C07K 2319/02 (2013.01)] 17 Claims
 
1. An expression construct comprising a polynucleotide sequence that encodes a protein operably linked to a recombinant secretion signal, wherein said recombinant secretion signal comprises a leader peptide comprising the amino acid sequence of SEQ ID NO: 1 or SEQ ID NO: 2 and a signal peptide comprising the amino acid sequence of SEQ ID NO: 127.