	US 12,324,842 B2
	Compositions and methods for making antibody conjugates
Andrew Tsourkas, Bryn Mawr, PA (US); and James Z Hui, Philadelphia, PA (US)
Assigned to THE TRUSTEES OF THE UNIVERSITY OF PENNSYLVANIA, Philadelphia, PA (US)
Filed by THE TRUSTEES OF THE UNIVERSITY OF PENNSYLVANIA, Philadelphia, PA (US)
Filed on Aug. 25, 2021, as Appl. No. 17/411,815.
Application 17/411,815 is a continuation of application No. 15/573,757, granted, now 11,123,440, previously published as PCT/US2016/032221, filed on May 12, 2016.
Claims priority of provisional application 62/160,130, filed on May 12, 2015.
Prior Publication US 2023/0046047 A1, Feb. 16, 2023
This patent is subject to a terminal disclaimer.
Int. Cl. A61K 47/68 (2017.01); A61K 39/00 (2006.01); C07K 14/315 (2006.01); C07K 16/28 (2006.01); C07K 16/46 (2006.01); C12N 9/48 (2006.01); C12N 15/62 (2006.01)

CPC A61K 47/6889 (2017.08) [A61K 47/6851 (2017.08); C07K 14/315 (2013.01); C07K 16/2803 (2013.01); C07K 16/2809 (2013.01); C07K 16/2887 (2013.01); C07K 16/468 (2013.01); C12N 9/48 (2013.01); C12N 15/62 (2013.01); C12Y 304/2207 (2013.01); A61K 2039/572 (2013.01); C07K 2317/31 (2013.01); C07K 2317/52 (2013.01); C07K 2317/622 (2013.01); C07K 2317/73 (2013.01); C07K 2317/92 (2013.01); C07K 2319/20 (2013.01); C07K 2319/21 (2013.01); C07K 2319/30 (2013.01); C07K 2319/60 (2013.01); C07K 2319/61 (2013.01); C07K 2319/70 (2013.01); C07K 2319/705 (2013.01)]

16 Claims

1. An adapter comprising an antibody binding domain (AbBD), wherein the AbBD is a variant of a Protein G subdomain comprising an amino acid sequence with at least 80% identity to the sequence set forth in SEQ ID NO: 2 and having a photo-reactive amino acid replacement and/or photo-reactive amino acid modification at A24, K28 or both of the sequence with at least 80% identity to the sequence set forth in SEQ ID NO: 2, and wherein the AbBD specifically binds and crosslinks to an immunoglobulin.