US 12,324,663 B2
Systems and methods for making and using sensors, probes, and other devices
Qimin Quan, Somerville, MA (US); Wooyoung Hong, Gyeonggi-do (KR); and Marko Loncar, Belmont, MA (US)
Assigned to President and Fellows of Harvard College, Cambridge, MA (US)
Appl. No. 15/310,253
Filed by President and Fellows of Harvard College, Cambridge, MA (US)
PCT Filed May 11, 2015, PCT No. PCT/US2015/030125
§ 371(c)(1), (2) Date Nov. 10, 2016,
PCT Pub. No. WO2015/175398, PCT Pub. Date Nov. 19, 2015.
Claims priority of provisional application 61/991,842, filed on May 12, 2014.
Prior Publication US 2017/0265788 A1, Sep. 21, 2017
Int. Cl. A61B 5/1459 (2006.01); A61B 5/01 (2006.01); A61B 5/05 (2021.01); A61B 5/145 (2006.01); A61B 5/1455 (2006.01); A61B 5/1473 (2006.01); B82B 1/00 (2006.01); B82B 3/00 (2006.01); G01H 9/00 (2006.01); G01N 15/14 (2024.01); G01N 15/1429 (2024.01); G01N 15/1434 (2024.01); G01N 21/552 (2014.01); G01N 21/64 (2006.01); G01N 21/65 (2006.01); G01N 33/53 (2006.01); G01N 33/543 (2006.01); G01N 15/01 (2024.01); G01N 15/10 (2006.01)
CPC A61B 5/1459 (2013.01) [A61B 5/01 (2013.01); A61B 5/05 (2013.01); A61B 5/14546 (2013.01); A61B 5/14556 (2013.01); A61B 5/14735 (2013.01); B82B 1/001 (2013.01); B82B 3/0019 (2013.01); G01H 9/00 (2013.01); G01N 15/1429 (2013.01); G01N 15/1434 (2013.01); G01N 15/1484 (2013.01); G01N 21/553 (2013.01); G01N 21/6428 (2013.01); G01N 21/65 (2013.01); G01N 33/5308 (2013.01); G01N 33/54373 (2013.01); A61B 5/1455 (2013.01); A61B 2562/0238 (2013.01); A61B 2562/028 (2013.01); A61B 2562/0285 (2013.01); A61B 2562/029 (2013.01); A61B 2562/125 (2013.01); G01N 15/01 (2024.01); G01N 2015/1006 (2013.01); G01N 2201/08 (2013.01)] 25 Claims
OG exemplary drawing
 
1. An article for inserting a particle into a cell, the article comprising:
a member configured to guide and/or direct light to analyze the cell, the member comprising a starting portion, an end portion configured for insertion into the cell while maintaining viability of the cell, and a tapered portion connecting the starting portion to the end portion, wherein (i) the starting portion has a substantially constant average cross-sectional diameter of at least about 100 micrometers and is configured for guiding the end portion into the cell, (ii) the end portion for insertion into the cell while maintaining viability of the cell has a substantially constant average cross-sectional diameter of less than 100 nm and a length of between about 750 micrometers and about 3 mm, and (iii) the tapered portion connecting the starting portion to the end portion has a length of no more than about 500 micrometers; and
at least one particle attached to the end portion of the member and configured to facilitate analysis of the cell by interacting with an analyte from the cell such that a characteristic of the analyte is determined by light propagated through the member, wherein (i) the at least one particle is positioned in an optically interrogatable position with respect to light transmitted through the member to the end portion, (ii) the at least one particle is attached to the end portion of the member via an adhesive positioned between the at least one particle and the end portion of the member, and (iii) the adhesive comprises at least one of a polymer or an epoxy,
wherein the member is formed by etching.