	US 12,002,546 B2
	Methods of determining sensitivity to photoperiod in cannabis
Gregory Baute, Comox (CA); Shumin Wang, Vancouver (CA); Jason Sawler, Centreville (CA); and Jose Celedon, Vancouver (CA)
Assigned to Aurora Cannabis Enterprises Inc., Vancouver (CA)
Filed by Aurora Cannabis Enterprises Inc.
Filed on Apr. 13, 2022, as Appl. No. 17/720,128.
Application 17/720,128 is a continuation of application No. 17/439,757, previously published as PCT/CA2021/050442, filed on Mar. 31, 2021.
Claims priority of application No. 3077823 (CA), filed on Apr. 1, 2020.
Prior Publication US 2022/0235425 A1, Jul. 28, 2022
This patent is subject to a terminal disclaimer.
Int. Cl. G16B 20/20 (2019.01); A01H 1/00 (2006.01); A01H 6/28 (2018.01); G16B 20/00 (2019.01); G16B 20/40 (2019.01)

CPC G16B 20/20 (2019.02) [A01H 1/1215 (2021.01); A01H 6/28 (2018.05); C12Q 2600/13 (2013.01); C12Q 2600/156 (2013.01); G16B 20/00 (2019.02); G16B 20/40 (2019.02)]

9 Claims

1. A method for producing a Cannabis plant having a day length neutral phenotype, or carrying a trait for a day length neutral phenotype, the method comprising:

producing a Cannabis plant having a day length neutral phenotype, comprising:

first, performing a cross of a first parent having at least one allele associated with a day length neutral phenotype, with a second parent that has a day length sensitive phenotype, to produce a first progeny plant,

second, identifying a first progeny plant from the first cross that has the day length neutral phenotype, or that is a carrier of a trait for the day length neutral phenotype,

third, performing a cross of a first progeny plant that has the day length neutral phenotype, or that is a carrier of a trait for the day length neutral phenotype, with a second first progeny plant that has the day length neutral phenotype, or that is a carrier of a trait for the day length neutral phenotype, to produce a second progeny plant, and

identifying a Cannabis plant having a day length neutral phenotype or carrying a trait for a day length neutral phenotype, comprising testing nucleic acid from the Cannabis plant to determine the presence or absence of an allele,

wherein the allele is in linkage disequilibrium r²=0.9 to 1 with a variation in a polymorphic site in the endogenous UPF2 gene

wherein the variation in a polymorphic site in the endogenous UPF2 gene corresponds to a polynucleotide with a sequence as set forth in SEQ ID NO: 2, and wherein the absence of SEQ ID NO: 2 or a portion thereof from the endogenous UPF2 gene is associated with the day length neutral phenotype, and

wherein the presence of the allele indicates that the plant has a day length neutral phenotype or is a carrier of the trait for the day length neutral phenotype.