US 12,000,838 B2
Reagents and methods for elemental mass spectrometry of biological samples
Alexander V. Loboda, Thornhill (CA); Olga Ornatsky, Richmond Hill (CA); Vladimir Baranov, Richmond Hill (CA); Nikita Zabinyakov, North York (CA); Anastasia Mavropoulos, Daly City (CA); Daniel Majonis, North York (CA); Bedilu Allo, Whitby (CA); and Taunia Closson, Markham (CA)
Assigned to STANDARD BIOTOOLS CANADA INC., Markham (CA)
Appl. No. 17/050,716
Filed by Fluidigm Canada Inc., Markham (CA)
PCT Filed Apr. 26, 2019, PCT No. PCT/US2019/029443
§ 371(c)(1), (2) Date Oct. 26, 2020,
PCT Pub. No. WO2019/210233, PCT Pub. Date Oct. 31, 2019.
Claims priority of provisional application 62/728,761, filed on Sep. 8, 2018.
Claims priority of provisional application 62/728,594, filed on Sep. 7, 2018.
Claims priority of provisional application 62/663,828, filed on Apr. 27, 2018.
Prior Publication US 2021/0239707 A1, Aug. 5, 2021
Int. Cl. G01N 33/68 (2006.01); C07K 1/13 (2006.01); G01N 1/28 (2006.01)
CPC G01N 33/6848 (2013.01) [C07K 1/13 (2013.01); G01N 1/28 (2013.01); G01N 2458/15 (2013.01); G01N 2560/00 (2013.01)] 20 Claims
OG exemplary drawing
 
1. A sample carrier for mass cytometry, the sample carrier comprising:
a planar surface, the planar surface including a first region for binding a sample;
a polydopamine layer disposed on the planar surface;
a self-assembled monolayer disposed on the polydopamine layer;
a 3D polymer brush attached to the self-assembled monolayer;
an element standard, the element standard comprising one or more elements or isotopes, wherein:
the element standard comprises an element film applied to a second region of the planar surface using an adhesive,
the element film is a polymer element film, and
the first region does not overlap with the second region.