	US 12,000,825 B2
	Method of selecting stem cells having ability to produce extracellular vesicles with high efficiency using activation of protease-activated receptor-mediated signaling pathways
Yun Sil Chang, Seoul (KR); Won Soon Park, Seoul (KR); and Dong Kyung Sung, Seoul (KR)
Assigned to SAMSUNG LIFE PUBLIC WELFARE FOUNDATION, Seoul (KR)
Appl. No. 16/961,851
Filed by SAMSUNG LIFE PUBLIC WELFARE FOUNDATION, Seoul (KR)
PCT Filed Apr. 28, 2020, PCT No. PCT/KR2020/005599 § 371(c)(1), (2) Date Jul. 13, 2020, PCT Pub. No. WO2020/222503, PCT Pub. Date Nov. 5, 2020.
Claims priority of application No. 10-2019-0049898 (KR), filed on Apr. 29, 2019; and application No. 10-2020-0050893 (KR), filed on Apr. 27, 2020.
Prior Publication US 2021/0255169 A1, Aug. 19, 2021
Int. Cl. G01N 33/50 (2006.01); C12N 5/0775 (2010.01); G01N 33/573 (2006.01)

CPC G01N 33/5023 (2013.01) [C12N 5/0665 (2013.01); G01N 33/573 (2013.01); C12N 2501/734 (2013.01); G01N 2333/974 (2013.01)]

6 Claims

1. A method of selecting a stem cell having an ability to produce extracellular vesicles with high efficiency, the method comprising measuring an activation level of a protease-activated receptor (PAR)-mediated signaling pathway,

wherein the PAR is PAR1 or PAR3,

wherein the ability to produce extracellular vesicles with high efficiency is promotion of the production of extracellular vesicles or promotion of a level of a protein in extracellular vesicles, and

wherein the measurement of the activation level of the PAR-mediated signaling pathway comprises the following processes:

(a) culturing stem cells and then treating the stem cells with thrombin;

(b) measuring an expression level of one or more selected from the group consisting of Ras-related protein Rab-5 (Rab-5), early endosome antigen-1 (EEA-1), phospho-extracellular signal-regulated kinase 1/2 (p-ERK1/2), and phospho-protein kinase B (p-AKT) in the thrombin-treated stem cells; and

(c) selecting a stem cell in which the expression level has been increased, as a stem cell having an ability to produce extracellular vesicles with high efficiency.