CPC C12Q 1/6869 (2013.01) [C07H 19/10 (2013.01); C07H 19/207 (2013.01); C07K 19/00 (2013.01); C09B 69/105 (2013.01)] | 43 Claims |
1. A labeled nucleotide analog comprising:
a first avidin protein having four subunits, each subunit comprising one biotin binding site;
a first nucleotide compound, bound to the first avidin protein, the first nucleotide compound comprising a polyphosphate element, a nucleoside element, an optional multivalent central core element, a terminal coupling element, and a nucleotide linker element; and
a first dye-labeled compound, bound to the first avidin protein;
wherein the first dye-labeled compound is a compound of structural formula (IIIG):
![]() wherein
each L′ is independently a dye compound linker element;
each S is independently a shield element;
each Dye is independently either an acceptor dye or a donor dye;
each B″ is independently a terminal coupling element;
each p is independently 0 or 1; and
each r″ is independently an integer from 0 to 8;
s is an integer from 1 to 6; and
t is 0 or 1;
wherein the compound of structural formula (IIIG) comprises at least one shield element,
at least one acceptor dye, and at least one donor dye; and
wherein the at least one shield element does not comprise a protein.
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