	US 11,999,992 B2
	Nucleic acid stabilization reagent, kits, and methods of use thereof
Jason M. McEwen, El Cerrito, CA (US)
Assigned to Bruker Cellular Analysis, Inc., Emeryville, CA (US)
Filed by Berkeley Lights, Inc., Emeryville, CA (US)
Filed on Sep. 13, 2018, as Appl. No. 16/130,634.
Application 16/130,634 is a continuation of application No. PCT/US2017/025065, filed on Mar. 30, 2017.
Claims priority of provisional application 62/316,514, filed on Mar. 31, 2016.
Prior Publication US 2019/0085375 A1, Mar. 21, 2019
Int. Cl. C12Q 1/6806 (2018.01); B01L 3/00 (2006.01); C12N 5/0781 (2010.01); C12N 5/0783 (2010.01); C12N 5/0786 (2010.01); C12Q 1/686 (2018.01)

CPC C12Q 1/6806 (2013.01) [B01L 3/5027 (2013.01); C12N 5/0635 (2013.01); C12N 5/0636 (2013.01); C12N 5/0645 (2013.01); C12N 5/0646 (2013.01); C12Q 1/686 (2013.01)]

21 Claims

1. A method of stabilizing a population of ribonucleic acids in a biological cell, wherein the biological cell is an animal cell, comprising:

contacting the animal cell with at least one irreversible protein translation inhibitor; at least one ribonucleic acid transcription inhibitor; and at least one electron transport chain agent comprising an electron transport chain inhibitor and/or an electron transport chain decoupling agent, wherein the at least one ribonucleic acid transcription inhibitor is a CDK9 inhibitor, aurethricin, thiolutin, amanitin, and/or triptolide; and/or the electron transport chain agent is rotenone, 2-thenoyltrifluoroacetone, carboxin, cyanide, sodium azide, 2,4 dinitrophenol, dicumarol, and/or carbonylcyanide-4-(trifluoromethoxy)-phenylhydrazone; and

wherein the contacting is performed for a period of time sufficient to retain the size distribution of the population of ribonucleic acids within the animal cell.