US 11,999,987 B2
Bacterial cocultures expressing a bacteriocin system
James L. Steele, Lebanon, NH (US); Brooks Henningsen, Salisbury, NH (US); Jeffery R. Broadbent, North Amalga, UT (US); Ekkarat Phrommao, Lebanon, NH (US); and Fernanda Cristina Firmino, Lebanon, NH (US)
Assigned to Lallemand Hungary Liquidity Management LLC, Budapest (HU)
Filed by Lallemand Hungary Liquidity Management LLC, Budapest (HU)
Filed on Mar. 19, 2021, as Appl. No. 17/206,943.
Claims priority of provisional application 62/991,850, filed on Mar. 19, 2020.
Prior Publication US 2021/0292803 A1, Sep. 23, 2021
Int. Cl. C12P 39/00 (2006.01); C07K 14/335 (2006.01); C12N 1/20 (2006.01); C12P 7/06 (2006.01)
CPC C12P 39/00 (2013.01) [C07K 14/335 (2013.01); C12N 1/20 (2013.01); C12P 7/06 (2013.01); C12Y 101/01001 (2013.01); C12Y 401/01001 (2013.01)] 22 Claims
 
1. A co-culture of lactic acid bacterial cells for making ethanol from a biomass, the co-culture comprising:
a first recombinant lactic acid bacteria (LAB) cell:
expressing at least one bacteriocin; and
comprising heterologous nucleic acid molecules encoding polypeptides for converting the biomass into ethanol;
and
a second recombinant LAB cell:
capable of converting the biomass into ethanol; comprising heterologous nucleic acid molecules encoding polypeptides for converting the biomass into ethanol;
and comprising one or more heterologous nucleic acid molecules encoding one or more polypeptides for conferring immunity against the at least one bacteriocin expressed by the first recombinant LAB cell
wherein the polypeptides for converting the biomass into ethanol comprise at least:
a pyruvate decarboxylase; and
an alcohol dehydrogenase,
wherein the at least one bacteriocin comprises a lantibiotic, a cyclic bacteriocin, a Class IIA bacteriocin, a Class IIB bacteriocin, or a combination thereof.