US 11,999,986 B2
Process for the conversion of lignocellulose material into an organic acid
Johan Pieter Marinus Sanders, Groningen (NL); and Robert Reurd Christophor Bakker, Wageningen (NL)
Assigned to PURAC BIOCHEM B.V., Gorinchem (NL)
Filed by PURAC BIOCHEM B.V., Gorinchem (NL)
Filed on Mar. 10, 2022, as Appl. No. 17/691,325.
Application 17/691,325 is a continuation of application No. 14/353,443, granted, now 11,279,964, previously published as PCT/NL2012/050735, filed on Oct. 23, 2012.
Claims priority of application No. 11186513 (EP), filed on Oct. 25, 2011.
Prior Publication US 2022/0195480 A1, Jun. 23, 2022
This patent is subject to a terminal disclaimer.
Int. Cl. C12P 21/06 (2006.01); C12P 7/46 (2006.01); C12P 7/54 (2006.01); C12P 7/56 (2006.01); C12P 13/04 (2006.01)
CPC C12P 21/06 (2013.01) [C12P 7/46 (2013.01); C12P 7/54 (2013.01); C12P 7/56 (2013.01); C12P 13/04 (2013.01); Y02P 20/129 (2015.11)] 14 Claims
 
1. A process for the conversion of lignocellulose into an organic acid, the process comprising the following steps:
a) pretreating a feed comprising lignocellulose material with an alkaline agent comprising a divalent cation, wherein the divalent cation is a calcium or magnesium cation, in the presence of water at a pretreatment temperature, to obtain an aqueous slurry of alkaline pretreated lignocellulose material;
b) supplying at least part of the slurry of alkaline pretreated lignocellulose material to a fermentation zone and subjecting the pretreated lignocellulose material, in the fermentation zone in the presence of a hydrolytic enzyme and a micro-organism that is able to convert saccharides into an organic acid, to enzymatic hydrolysis and fermentation to obtain a fermentation broth comprising insoluble lignocellulose, precipitated and dissolved salt of the organic acid with the divalent cation, and enzyme;
c) discharging fermentation broth obtained in step b) from the fermentation zone;
d) separating from the discharged fermentation broth a liquid phase comprising the dissolved salt of the organic acid and a solid phase comprising insoluble lignocellulose and the precipitated salt of the organic acid, washing the separated solid phase with water at a temperature in the range of from 10 to 50° C. to obtain a washed solid phase and wash water; and supplying at least part of the wash water to step a);
e) recycling at least part of the liquid phase separated in step d) to said alkaline pre-treatment step a) and/or to the fermentation zone; and,
f) recovering the salt of the organic acid from the washed solid phase separated in step d) by extracting said washed solid phase with water at a temperature in the range of from 60 to 100° C. to obtain a solution of the salt of the organic acid and an extracted solid phase.