	US 11,999,982 B2
	Method for the fermentative production of guanidinoacetic acid
Frank Schneider, Halle (DE); and Frank Jankowitsch, Sassenberg (DE)
Assigned to Evonik Operations GmbH, Essen (DE)
Appl. No. 18/004,364
Filed by Evonik Operations GmbH, Essen (DE)
PCT Filed Jun. 28, 2021, PCT No. PCT/EP2021/067647 § 371(c)(1), (2) Date Jan. 5, 2023, PCT Pub. No. WO2022/008276, PCT Pub. Date Jan. 13, 2022.
Claims priority of application No. 20184966 (EP), filed on Jul. 9, 2020.
Prior Publication US 2023/0265471 A1, Aug. 24, 2023
This patent is subject to a terminal disclaimer.
Int. Cl. C12P 13/00 (2006.01); C12N 1/21 (2006.01); C12N 9/10 (2006.01); C12N 15/52 (2006.01)

CPC C12P 13/00 (2013.01) [C12N 9/1003 (2013.01); C12N 9/1022 (2013.01); C12N 9/1096 (2013.01); C12N 15/52 (2013.01); C12Y 201/04001 (2013.01); C12Y 203/03009 (2013.01); C12Y 206/01002 (2013.01); C12Y 206/01004 (2013.01); C12Y 206/01044 (2013.01)]

28 Claims

1. A microorganism, comprising:

at least one gene coding for a protein having a function of a L-arginine:glycine amidinotransferase, and wherein an activity of a protein having a function of a malate synthase is decreased compared to the respective activity in a corresponding wildtype microorganism,

wherein the protein having the function of the L-arginine:glycine amidinotransferase comprises an amino acid sequence which is at least 80% identity to the amino acid sequence according to SEQ ID NO: 11.