CPC C12N 9/22 (2013.01) [C12N 9/96 (2013.01); G01N 1/4044 (2013.01)] | 15 Claims |
1. A method of preparing a cell suspension, comprising:
(a) obtaining a recombinant nuclease,
(b) incubating the cell suspension with the recombinant nuclease until nucleic acids present in the suspension and/or released from cells are removed, and then
(c) flowing the cells through a microfluidic channel;
wherein the recombinant nuclease comprises an amino acid sequence that is at least 90% identical to the amino acid sequence set forth in SEQ ID NO:2,
wherein the recombinant nuclease when present in a solution at 4° C. has non-specific nuclease (NSN) activity with a kcat value of at least 10 s−1, and
wherein removing nucleic acids from the suspension in step (b) prevents nucleic acids from clogging the microfluidic channel in step (c).
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