CPC C12N 9/0006 (2013.01) [C12N 11/02 (2013.01); C12P 7/22 (2013.01); C12P 7/62 (2013.01); C12P 41/002 (2013.01); C12Y 101/01184 (2013.01)] | 19 Claims |
1. An engineered ketoreductase polypeptide which is, under suitable reaction conditions, capable of catalyzing the conversion of 4-hydroxy-2-butanone to (R) -(-)-1,3-butanediol, wherein the amino acid sequence of said polypeptide comprises one or more of substitutions selected from the group consisting of substitutions corresponding to substitutions X46T, X185C, and X228A in the polypeptide of SEQ ID NO 2, wherein the numbering refers to SEQ ID NO:2, wherein said amino acid sequence is selected from the group consisting of SEQ ID NO: 36, 38, 40, 42, 44, 46, 48, 50, 52, 54, 56, 58, 60, 62, 66, 68, 70, 72, 74, 78, 80, 82, 84, 86, 88, 90, 92, 94, 96, 98, 100, 102, 104, 106, 108, 126, 128, 130, 132, 134, 136, 138, 140, 142, 144, 146, 148, 150, 152, 154, 156, 158, 160, 162, 164, 166, 168, 170, 172, 174, 176, 178, 180, 182, 184, 186, 188, 190, 192, 194, 196, 198, 200, 202, 204, 206, 208, 210, 212, 214, 216, 218, 220, 222, 224, 226, 228, 230, 232, 234, 236, 238, 240, 242, 244, 246, 248, 250, 252, 254, 256, 258, 260, 262, 264, 266, 268, 270, 272, 274, 276, 278, 280, 282, 284, 286, 288, 290, 292, 294, 296, 298, 300, 302, 304, 306, 308, 310, 312, 314, 316, 318, 320, 322, 324, 326, 328, 330, and 332.
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