	US 11,999,975 B2
	Method for purifying an enveloped virus
Volker Sandig, Berlin (DE); Michael Mühle, Berlin (DE); and Sven Krügener, Berlin (DE)
Assigned to ProBioGen AG, Berlin (DE)
Appl. No. 17/256,158
Filed by ProBioGen AG, Berlin (DE)
PCT Filed Jun. 27, 2019, PCT No. PCT/EP2019/067216 § 371(c)(1), (2) Date Dec. 24, 2020, PCT Pub. No. WO2020/007715, PCT Pub. Date Jan. 9, 2020.
Claims priority of application No. 18181748 (EP), filed on Jul. 4, 2018.
Prior Publication US 2022/0267738 A1, Aug. 25, 2022
Int. Cl. C07K 1/22 (2006.01); B01D 15/32 (2006.01); B01D 15/36 (2006.01); B01D 15/38 (2006.01); C07K 1/16 (2006.01); C07K 1/18 (2006.01); C07K 1/36 (2006.01); C12N 7/00 (2006.01); C12N 15/113 (2010.01)

CPC C12N 7/00 (2013.01) [B01D 15/327 (2013.01); B01D 15/361 (2013.01); B01D 15/3847 (2013.01); C12N 15/113 (2013.01); C12N 2710/24151 (2013.01); C12N 2760/18151 (2013.01)]

7 Claims

1. A method for purifying an enveloped virus comprising the steps of:

(i) binding an enveloped virus comprised in a preparation to a mixed mode chromatography carrier, and

(ii) eluting the enveloped virus from the mixed mode chromatography carrier with an elution buffer, wherein the mixed mode chromatography carrier is a hydrophobic ion exchange chromatography carrier and wherein the elution buffer comprises arginine.