US 11,999,972 B2
Fibronectin fragment to be used for stem cell production
Tomomi Otsuji, Kusatsu (JP); Toshikazu Nishie, Kusatsu (JP); Risa Kato, Kusatsu (JP); Sachiko Okamoto, Kusatsu (JP); Tatsuji Enoki, Kusatsu (JP); and Junichi Mineno, Kusatsu (JP)
Assigned to TAKARA BIO INC., Shiga (JP)
Appl. No. 16/321,112
Filed by TAKARA BIO INC., Shiga (JP)
PCT Filed Jul. 28, 2017, PCT No. PCT/JP2017/027485
§ 371(c)(1), (2) Date May 6, 2019,
PCT Pub. No. WO2018/021543, PCT Pub. Date Feb. 1, 2018.
Claims priority of application No. 2016-149495 (JP), filed on Jul. 29, 2016.
Prior Publication US 2021/0254018 A1, Aug. 19, 2021
Int. Cl. C12N 5/074 (2010.01); A61K 38/39 (2006.01); C07K 14/78 (2006.01); C12M 1/00 (2006.01); C12M 1/12 (2006.01)
CPC C12N 5/0696 (2013.01) [A61K 38/39 (2013.01); C07K 14/78 (2013.01); C12M 25/06 (2013.01); C12M 23/20 (2013.01); C12N 2533/52 (2013.01)] 6 Claims
 
1. A recombinant polypeptide comprising in the same molecule:
(a) a polypeptide comprising three repeats selected from the group consisting of human fibronectin III-1 to 7 repeats wherein the III-1 to 7 repeats comprise the amino acid sequences of SEQ ID NO: 1, 2, 3, 4, 5, 6, and 7 respectively, or a variant of the polypeptide comprising three repeats selected from the group consisting of human fibronectin III-1 to 7 repeats wherein the III-1 to 7 repeats comprise an amino acid sequence having at least 90% identity to the amino acid sequence of SEQ ID NO: 1, 2, 3, 4, 5, 6, and 7 respectively;
(b) a polypeptide comprising human fibronectin III-8 to 10 repeats comprising the amino acid sequences of SEQ ID NO: 8, 9 and 10 respectively, or a variant of the polypeptide comprising human fibronectin III-8 to 10 repeats comprising an amino acid sequence having at least 90% identity to the amino acid sequence of SEQ ID NO: 8, 9 and 10 respectively; and
(c) a polypeptide comprising human fibronectin III-12 to 14 repeats comprising the amino acid sequences of SEQ ID NO: 12, 13 and 14 respectively, or a variant of the polypeptide comprising human fibronectin III-12 to 14 repeats comprising an amino acid sequence having at least 90% identity to the amino acid sequence of SEQ ID NO: 12, 13 and 14 respectively;
wherein the recombinant polypeptide retains a function of maintaining an undifferentiated state of stem cells, and
wherein the molecular weight of the recombinant polypeptide is 100 kDa or less.