US 11,999,966 B2
Apparatuses and methods using nanostraws to deliver biologically relevant cargo into non-adherent cells
Karl Martin Hjort, Lund (SE); Sergio Leal-Ortiz, Hayward, CA (US); Yuhong Cao, Palo Alto, CA (US); Chris Rehse, Palo Alto, CA (US); Andy Kah Ping Tay, Stanford, CA (US); and Nicholas A. Melosh, Menlo Park, CA (US)
Assigned to The Board of Trustees of the Leland Stanford Junior University, Stanford, CA (US)
Filed by The Board of Trustees of the Leland Stanford Junior University, Stanford, CA (US)
Filed on Oct. 27, 2020, as Appl. No. 17/081,983.
Application 17/081,983 is a continuation of application No. 16/038,062, filed on Jul. 17, 2018, granted, now 10,815,499.
Claims priority of provisional application 62/534,511, filed on Jul. 19, 2017.
Prior Publication US 2021/0062222 A1, Mar. 4, 2021
Int. Cl. C12N 15/87 (2006.01); B82Y 5/00 (2011.01); B82Y 40/00 (2011.01); C12M 1/00 (2006.01); C12M 1/42 (2006.01); C12M 3/00 (2006.01); C12M 3/06 (2006.01); C12N 5/00 (2006.01)
CPC C12N 15/87 (2013.01) [C12M 23/02 (2013.01); C12M 23/42 (2013.01); C12M 35/00 (2013.01); C12M 35/02 (2013.01); C12M 35/04 (2013.01); B82Y 5/00 (2013.01); B82Y 40/00 (2013.01); C12M 23/16 (2013.01); C12N 5/0068 (2013.01); C12N 5/0075 (2013.01); C12N 2521/00 (2013.01); C12N 2535/00 (2013.01)] 10 Claims
OG exemplary drawing
 
1. A flow-through apparatus for delivering a biologically relevant cargo into non-adherent cells, the apparatus comprising:
a first flow path having an inlet port and an outlet port, wherein the first flow path is configured to pass a suspension of cells from the inlet port to the outlet port;
a deflectable substrate along a lateral side of the first flow path;
a plurality of nanostraws extending through the deflectable substrate and into the first flow path, wherein the plurality of nanostraws extend from the flexible substrate, further wherein the plurality of nanostraws are hollow and have an inner diameter of between 5 nm-1500 nm;
a reservoir in fluid communication with an inside of the plurality of nanostraws, the reservoir configured to hold the biologically relevant cargo;
wherein the deflectable substrate is configured to deflect between a first position and a second position, further wherein the plurality of nanostraws extend further into the first flow path in the second position than in the first position to at least partially occlude the first flow path so that non-adherent cell in the flow path communicate with one or more of the plurality of nanostraws.