	US 11,999,964 B2
	Synthetic mammalian signaling circuits for robust cell population control
Yitong Ma, Pasadena, CA (US); Mark W. Budde, Pasadena, CA (US); Michaelle N. Mayalu, Pasadena, CA (US); and Michael B. Elowitz, Pasadena, CA (US)
Assigned to California Institute of Technology, Pasadena, CA (US)
Filed by California Institute of Technology, Pasadena, CA (US)
Filed on Aug. 27, 2021, as Appl. No. 17/460,120.
Claims priority of provisional application 63/072,014, filed on Aug. 28, 2020.
Prior Publication US 2022/0064666 A1, Mar. 3, 2022
Int. Cl. C12N 15/85 (2006.01); C07K 14/415 (2006.01); C12N 9/02 (2006.01); C12N 9/86 (2006.01); C12N 15/11 (2006.01)

CPC C12N 15/85 (2013.01) [C07K 14/415 (2013.01); C12N 9/0069 (2013.01); C12N 9/86 (2013.01); C12N 15/11 (2013.01); C12Y 113/12003 (2013.01); C07K 2319/00 (2013.01); C12N 2310/20 (2017.05); C12N 2800/107 (2013.01); C12N 2810/10 (2013.01)]

11 Claims

1. A nucleic acid composition, comprising:

a first polynucleotide encoding a first synthase, wherein the first synthase comprises an indole-3 acetic acid hydrolase capable of catalyzing the synthesis of an orthogonal signal from a first precursor molecule;

a second polynucleotide encoding a second synthase, wherein the second synthase is capable of catalyzing the synthesis of the first precursor molecule from a second precursor molecule; and

a third polynucleotide encoding a transporter capable of transporting the orthogonal signal across a cell membrane;

wherein the first polynucleotide, the second polynucleotide, and the third polynucleotide are operably linked to a ubiquitous mammalian promoter.