	US 11,999,959 B2
	Binary vectors and uses of same
Fabio Pasin, Madrid (ES); Juan Antonio García Álvarez, Madrid (ES); Leonor Cecilia Bedoya Rojas, Madrid (ES); Maria del Carmen Simón Mateo, Madrid (ES); Diego Vicente Orzáez Calatayud, Madrid (ES); and Juan Miguel Bernabé Orts, Madrid (ES)
Assigned to Juan Antonio García Álvarez, Madrid (ES)
Filed by Juan Antonio García Álvarez, Madrid (ES)
Filed on Feb. 26, 2023, as Appl. No. 18/114,264.
Application 18/114,264 is a continuation of application No. 16/622,037, granted, now 11,597,939, previously published as PCT/ES2018/070421, filed on Jun. 12, 2018.
Claims priority of application No. 201730792 (ES), filed on Jun. 12, 2017.
Prior Publication US 2023/0365983 A1, Nov. 16, 2023
This patent is subject to a terminal disclaimer.
Int. Cl. C12N 15/82 (2006.01)

CPC C12N 15/8205 (2013.01) [C12N 15/8241 (2013.01)]

20 Claims

1. A binary vector comprising a nucleic acid sequence comprising in this order:

(a) a T-DNA right border comprising a nucleotide sequence selected from the group consisting of SEQ ID NO: 102 and SEQ ID NO: 115; and

(b) a sequence portion connecting item (a) with the following item (c); and

(d) an origin of replication that is functional in at least one bacterial species; and

(e) a bacterial terminator; and

(f) a sequence portion connecting item (e) with the following item (g); and

(g) a T-DNA left border comprising a nucleotide sequence selected from the group consisting of SEQ ID NO: 103, SEQ ID NO: 104 and SEQ ID NO: 116; and

wherein the bacterial terminator comprises a nucleotide sequence selected from the group consisting of SEQ ID NO: 108, SEQ ID NO: 109, SEQ ID NO: 110 and SEQ ID NO: 111; and

wherein the sequence of item (b) and item (f) has a size of less than 200 nucleotides.