CPC C12N 15/1093 (2013.01) [Y02P 20/582 (2015.11)] | 14 Claims |
1. A method for preparing a sequencing library, comprising:
(a) contacting a target DNA molecule with a transposase, resulting in multiple insertions of a continuous transposon to produce a modified target DNA molecule, wherein the continuous transposon comprises an adaptor domain;
(b) amplifying fragments of the target DNA molecule by contacting the modified target DNA molecule with oligonucleotide primers that anneal to the adaptor domain, wherein each oligonucleotide primer comprises the same compartment-specific barcode sequence; and
(c) before step (b), performing one of the following:
(i) prior to step (a), compartmentalizing the target DNA molecule, or
(ii) after step (a), compartmentalizing the modified target DNA molecule;
wherein steps (a), (b), and (c) create a plurality of tagged target DNA fragments each comprising an identical or complementary barcode sequence.
|