US 11,999,951 B2
Massively parallel contiguity mapping
Jay Ashok Shendure, Seattle, WA (US); Jerrod Joseph Schwartz, Seattle, WA (US); Andrew Colin Adey, Seattle, WA (US); Cho li Lee, Seattle, WA (US); Joseph Brian Hiatt, Seattle, WA (US); Jacob Otto Kitzman, Seattle, WA (US); and Akash Kumar, Seattle, WA (US)
Assigned to University of Washington through its Center for Commercialization, Seattle, WA (US)
Filed by University of Washington Through Its Center for Commercialization, Seattle, WA (US)
Filed on Apr. 8, 2022, as Appl. No. 17/716,539.
Application 17/716,539 is a division of application No. 16/665,800, filed on Oct. 28, 2019, granted, now 11,299,730, issued on Apr. 12, 2022.
Application 16/665,800 is a continuation of application No. 13/513,309, granted, now 10,457,936, issued on Oct. 29, 2019, previously published as PCT/US2012/023679, filed on Feb. 2, 2012.
Claims priority of provisional application 61/473,083, filed on Apr. 7, 2011.
Claims priority of provisional application 61/438,935, filed on Feb. 2, 2011.
Prior Publication US 2022/0315918 A1, Oct. 6, 2022
This patent is subject to a terminal disclaimer.
Int. Cl. C12N 15/10 (2006.01)
CPC C12N 15/1093 (2013.01) [Y02P 20/582 (2015.11)] 14 Claims
 
1. A method for preparing a sequencing library, comprising:
(a) contacting a target DNA molecule with a transposase, resulting in multiple insertions of a continuous transposon to produce a modified target DNA molecule, wherein the continuous transposon comprises an adaptor domain;
(b) amplifying fragments of the target DNA molecule by contacting the modified target DNA molecule with oligonucleotide primers that anneal to the adaptor domain, wherein each oligonucleotide primer comprises the same compartment-specific barcode sequence; and
(c) before step (b), performing one of the following:
(i) prior to step (a), compartmentalizing the target DNA molecule, or
(ii) after step (a), compartmentalizing the modified target DNA molecule;
wherein steps (a), (b), and (c) create a plurality of tagged target DNA fragments each comprising an identical or complementary barcode sequence.