US 11,999,945 B2
Modulating polymer beads for DNA processing
Steven Norberg, La Mesa, CA (US); Dmitry K. Pokholok, San Diego, CA (US); Ramesh Ramji, San Diego, CA (US); Frank J. Steemers, Encinitas, CA (US); and Fan Zhang, San Diego, CA (US)
Assigned to Illumina, Inc., San Diego, CA (US)
Filed by Illumina, Inc., San Diego, CA (US)
Filed on Jul. 1, 2021, as Appl. No. 17/305,230.
Application 17/305,230 is a continuation of application No. 16/663,060, filed on Oct. 24, 2019, granted, now 11,085,036.
Claims priority of provisional application 62/704,028, filed on Oct. 26, 2018.
Prior Publication US 2021/0332349 A1, Oct. 28, 2021
This patent is subject to a terminal disclaimer.
Int. Cl. C12N 15/10 (2006.01); B01L 3/00 (2006.01); C40B 30/00 (2006.01); C40B 60/02 (2006.01); C40B 80/00 (2006.01)
CPC C12N 15/1006 (2013.01) [B01L 3/502761 (2013.01); C12N 15/1065 (2013.01); C40B 30/00 (2013.01); C40B 60/02 (2013.01); C40B 80/00 (2013.01)] 24 Claims
 
1. A solid support comprising a plurality of beads, wherein each bead comprises:
a hydrogel polymer precursor;
a crosslinker; and
a biomolecule disposed within each bead, wherein each bead is a porous hollow bead that comprises multiple polymer layers, wherein each polymer layer comprises at least one of a distinct pore size and a distinct pore density that allows diffusion of a reagent through the bead while retaining the biomolecule.