US 11,999,939 B2
Process and system for propagating cell cultures while preventing lactate accumulation
Brandon John Downey, Visp (CH); John Michael Schmitt, Visp (CH); and Jeffrey Francis Breit, Visp (CH)
Assigned to LONZA LTD, Visp (CH)
Filed by LONZA LTD, Visp (CH)
Filed on Feb. 13, 2023, as Appl. No. 18/168,084.
Application 18/168,084 is a continuation of application No. 16/765,346, granted, now 11,634,680, previously published as PCT/US2018/061912, filed on Nov. 20, 2018.
Claims priority of provisional application 62/747,311, filed on Oct. 18, 2018.
Claims priority of provisional application 62/588,464, filed on Nov. 20, 2017.
Prior Publication US 2023/0279330 A1, Sep. 7, 2023
This patent is subject to a terminal disclaimer.
Int. Cl. C12M 1/34 (2006.01); C12M 1/36 (2006.01)
CPC C12M 41/32 (2013.01) [C12M 41/48 (2013.01)] 11 Claims
OG exemplary drawing
 
1. A process for propagating a cell culture comprising:
determining a concentration of at least one quality attribute in a cell culture;
measuring values of at least two attribute influencing parameters within the cell culture;
sending the concentration of the at least one quality attribute and the values of the attribute influencing parameter measurements to a controller, the controller including a predictive model that is configured to calculate a future concentration of the at least one quality attribute in the cell culture based at least in part on the values of the attribute influencing parameters;
running simulations on an optimizer to calculate future concentrations of the at least one quality attribute based on manipulation of the attribute influencing parameters within the cell culture; and
the controller selectively changing at least one condition within the cell culture based upon the determined future concentration of the at least one quality attribute in the cell culture for maintaining the at least one quality attribute concentration within preset limits.