US 11,999,829 B2
Method of making a polymersome
Jean-Christophe Leroux, Zurich (CH); Simon Matoori, Zurich (CH); and Olha Voznyuk Wuerthinger, Zurich (CH)
Assigned to ETH ZURICH, Zurich (CH)
Filed by ETH ZURICH, Zurich (CH)
Filed on Mar. 20, 2023, as Appl. No. 18/123,905.
Application 18/123,905 is a division of application No. 16/645,763, granted, now 11,713,376, previously published as PCT/IB2018/056887, filed on Sep. 10, 2018.
Claims priority of provisional application 62/557,256, filed on Sep. 12, 2017.
Prior Publication US 2023/0220165 A1, Jul. 13, 2023
Int. Cl. C08G 81/02 (2006.01); A61K 9/127 (2006.01); B01J 13/08 (2006.01); B01J 13/12 (2006.01); C08K 5/19 (2006.01); C08K 5/353 (2006.01); C08K 5/42 (2006.01); C09K 11/06 (2006.01); G01N 33/68 (2006.01); G01N 33/84 (2006.01)
CPC C08G 81/025 (2013.01) [B01J 13/08 (2013.01); B01J 13/12 (2013.01); C08K 5/19 (2013.01); C08K 5/353 (2013.01); C08K 5/42 (2013.01); C09K 11/06 (2013.01); G01N 33/689 (2013.01); G01N 33/84 (2013.01); A61K 9/1273 (2013.01); B01J 13/125 (2013.01); G01N 2800/085 (2013.01)] 14 Claims
OG exemplary drawing
 
1. A method of making a polymersome comprising (a) a membrane, which comprises a block copolymer of poly(styrene) (PS) and poly(ethylene oxide) (PEO), wherein the PS/PEO molecular weight ratio is higher than 1.0 and lower than 4.0; and (b) a core which encloses an acid and at least one pH-sensitive dye, comprising:
(a) dissolving the block copolymer of PS and PEO in an organic solvent, to form a copolymer-containing organic phase;
(b) mixing the copolymer-containing organic solvent phase with an aqueous phase containing the acid and the at least one pH-sensitive dye so as to form the polymersome; and
(c) removing the unencapsulated at least one pH-sensitive dye and organic solvent.