US 11,999,768 B2
Mutant Uracil DNA glycosylase with improved thermal sensitivity
Yong Keol Shin, Daejeon (KR); Jeongyeon Yoon, Daejeon (KR); Ki Hoon Nam, Daejeon (KR); Seulki Lim, Daejeon (KR); Wookyung Yu, Daegu (KR); Seongjun Park, Gyeongsangnam-do (KR); Juhwan Lee, Daegu (KR); Sangyeol Kim, Gyeongsangnam-do (KR); Moo Seok Kang, Busan (KR); and Minjae Seo, Daegu (KR)
Assigned to Enzynomics Co. Ltd., Daejeon (KR); and Daegu Gyeongbuk Institute of Science and Technology, Daegu (KR)
Appl. No. 17/264,509
Filed by ENZYNOMICS CO. LTD., Daejeon (KR); and DAEGU GYEONGBUK INSTITUTE OF SCIENCE AND TECHNOLOGY, Daegu (KR)
PCT Filed Oct. 22, 2020, PCT No. PCT/KR2020/014468
§ 371(c)(1), (2) Date Jan. 29, 2021,
PCT Pub. No. WO2022/085818, PCT Pub. Date Apr. 28, 2022.
Prior Publication US 2022/0396602 A1, Dec. 15, 2022
Int. Cl. C07K 14/245 (2006.01); C12N 15/63 (2006.01); C12Q 1/6848 (2018.01); C12Q 1/686 (2018.01)
CPC C07K 14/245 (2013.01) [C12N 15/63 (2013.01); C12Q 1/6848 (2013.01); C12Q 1/686 (2013.01)] 11 Claims
OG exemplary drawing
 
1. An isolated UDG (Uracil DNA Glycosylase) polypeptide from Escherichia coli consisting of the amino acid sequence set forth in SEQ ID NO: 1 having at least one amino acid substitution selected from the group consisting of E4A, W7A, E13A, Q16A, Y19A, D43X, F48A, F50A, E52A, H67A, K57A, Q71A, H73A, P87A, L96A, E112A, L121A, H134A, E142A, F144A, R156A, F161A, L162A, W164A, H180A, L183A, H202A, G214E, G214W, G214R, W220A, and L224A, in which the number indicates the position of the substituted amino acid and the amino acids are indicated as a single letter code and X indicates any amino acids, in which the codes on the left and right sides of the position indicate a wild type and substituted residues, respectively,
wherein the UDG has an improved thermal sensitivity in comparison to a wild type UDG from E. coli,
wherein the X of D43X is A, C, G, K, H, I, P, R, V or W.