US 11,999,666 B2
Use of lipo-chitooligosaccharides and/or chitooligosaccharides in combination with phosphate-solubilizing microorganisms to enhance plant growth
Michael Frodyma, Roanoke, VA (US); Shawn Semones, Salem, VA (US); R. Stewart Smith, Pewaukee, WI (US); and Yaowei Kang, Christiansburg, VA (US)
Assigned to Novozymes BioAg A/S, Bagsvaerd (DK)
Appl. No. 14/345,057
Filed by Michael Frodyma, Roanoke, VA (US); Shawn Semones, Salem, VA (US); R. Stewart Smith, Pewaukee, WI (US); and Yaowei Kang, Christiansburg, VA (US)
PCT Filed Sep. 14, 2012, PCT No. PCT/US2012/055435
§ 371(c)(1), (2), (4) Date Apr. 10, 2014,
PCT Pub. No. WO2013/040366, PCT Pub. Date Mar. 21, 2013.
Claims priority of provisional application 61/534,639, filed on Sep. 14, 2011.
Prior Publication US 2014/0342904 A1, Nov. 20, 2014
This patent is subject to a terminal disclaimer.
Int. Cl. C05F 11/08 (2006.01); A01N 43/16 (2006.01); A01N 63/36 (2020.01)
CPC C05F 11/08 (2013.01) [A01N 43/16 (2013.01); A01N 63/36 (2020.01)] 29 Claims
 
1. A method, comprising:
a) treating a corn seed with an inoculum comprising at least 1×107 spores of Penicillium bilaiae strain ATCC 20851, Penicillium bilaiae strain NRRL 50169, Penicillium bilaiae strain ATCC 22348, Penicillium bilaiae strain ATCC 18309, and/or Penicillium bilaiae strain NRRL 50162 per gram of inoculum; and
b) treating said corn seed with at least one lipo-chitooligosaccharide (LCO) and/or at least one chitooligosaccharide (CO) at a concentration ranging from about 10−14 to about 10−5 M,
wherein said corn seed is treated with said inoculum and said at least one LCO and/or at least one CO at least two months prior to planting.