US 11,998,914 B2
Micro-fluidic devices for assaying biological activity
Kevin T. Chapman, Santa Monica, CA (US); Daniele Malleo, El Cerrito, CA (US); J. Tanner Nevill, El Cerrito, CA (US); Steven W. Short, Pleasanton, CA (US); Mark P. White, San Francisco, CA (US); and M. Jimena Loureiro, Albany, CA (US)
Assigned to BRUKER CELLULAR ANALYSIS, INC., Emeryville, CA (US)
Filed by Berkeley Lights, Inc., Emeryville, CA (US)
Filed on Mar. 24, 2022, as Appl. No. 17/656,244.
Application 17/656,244 is a continuation of application No. 16/509,166, filed on Jul. 11, 2019, granted, now 11,305,283.
Application 16/509,166 is a continuation of application No. 15/843,122, filed on Dec. 15, 2017, granted, now 10,376,886, issued on Aug. 13, 2019.
Application 15/843,122 is a continuation of application No. 14/521,447, filed on Oct. 22, 2014, granted, now 9,889,445, issued on Feb. 13, 2018.
Application 14/521,447 is a continuation in part of application No. 14/520,510, filed on Oct. 22, 2014, granted, now 9,617,145, issued on Apr. 11, 2017.
Claims priority of provisional application 62/058,658, filed on Oct. 1, 2014.
Claims priority of provisional application 61/996,969, filed on Oct. 22, 2013.
Claims priority of provisional application 61/996,962, filed on Oct. 22, 2013.
Claims priority of provisional application 61/996,973, filed on Oct. 22, 2013.
Prior Publication US 2022/0388003 A1, Dec. 8, 2022
Int. Cl. B01L 3/00 (2006.01); B03C 5/00 (2006.01); B03C 5/02 (2006.01); G01N 15/14 (2006.01); G01N 33/50 (2006.01); G01N 33/543 (2006.01); G01N 33/68 (2006.01)
CPC B01L 3/502761 (2013.01) [B03C 5/005 (2013.01); B03C 5/026 (2013.01); G01N 15/1484 (2013.01); G01N 33/5023 (2013.01); G01N 33/5047 (2013.01); G01N 33/505 (2013.01); G01N 33/5052 (2013.01); G01N 33/54313 (2013.01); G01N 33/6854 (2013.01); B01L 2200/0647 (2013.01); B01L 2200/0668 (2013.01); B01L 2300/0636 (2013.01); B01L 2300/0816 (2013.01); B01L 2300/0864 (2013.01); B01L 2300/087 (2013.01); B01L 2400/0454 (2013.01); B03C 2201/26 (2013.01)] 28 Claims
OG exemplary drawing
 
1. A process of assaying biological activity of one or more biological cells in a holding pen of a micro-fluidic device, wherein said biological activity comprises production of a biological material of interest; and
wherein said micro-fluidic device comprises:
a base and a micro-fluidic structure disposed on said base;
wherein said micro-fluidic structure and said base define a flow region and said holding pen, and wherein said holding pen comprises a single opening to said flow region and an unswept region;
said process comprising:
introducing a capture micro-object into said holding pen, wherein said capture object comprises a binding substance that specifically binds to said biological material of interest;
allowing said biological material of interest produced by said one or more biological cells to bind to said capture micro-object; and
assessing whether said biological material of interest is bound to said capture micro-object.