CPC B01L 3/502738 (2013.01) [B25J 9/1697 (2013.01); B01L 2300/0636 (2013.01); B01L 2300/0883 (2013.01); B01L 2400/02 (2013.01); B01L 2400/049 (2013.01); B01L 2400/0666 (2013.01)] | 10 Claims |
1. A sample-to-droplet microfluidic system, comprising:
a sample-to-droplet microfluidic chip;
a valve control system connected to said sample-to-droplet microfluidic chip;
a vacuum system fluidly connected to said sample-to-droplet microfluidic chip; and
a droplet formation pressure system fluidly connected to said sample-to-droplet microfluidic chip,
wherein said sample-to-droplet microfluidic chip comprises:
a sample-to-droplet interface section;
a droplet mixing section in fluid connection with said sample-to-droplet interface section to received droplets therefrom;
an incubation section in fluid connection with said droplet mixing section to receive droplets therefrom; and
a detection section in fluid connection with said incubation section to receive droplets therefrom;
wherein said sample-to-droplet interface section comprises:
a first sample input channel with a first open port configured to receive a sample input,
a vacuum channel fluidly connected to said first sample input channel when a pair of intermediate valves therein are open, a first droplet forming region being between said pair of intermediate valves,
a first fluid pressure channel arranged to intercept said first droplet forming region with a valve prior to said first droplet forming region, said first fluid pressure channel further intercepting a droplet channel with a valve therebetween, said droplet channel leading to said droplet mixing section,
wherein said sample-to-droplet interface section further comprises:
a second sample input channel with an open port configured to receive a sample input, said vacuum channel being further fluidly connected to said second sample input channel when a pair of intermediate valves therein are open, a second droplet forming region being between said pair of intermediate valves, and
a second fluid pressure channel arranged to intercept said second droplet forming region with a valve prior to said droplet forming region, said second fluid pressure channel further intercepting said droplet channel with a valve therebetween.
|