US 11,998,907 B2
Cartridges, systems and methods for mass spectrometry
Nicholas Edward Manicke, Zionsville, IN (US); and Chengsen Zhang, Indianapolis, IN (US)
Assigned to The Trustees of Indiana University, Bloomington, IN (US)
Appl. No. 17/046,573
Filed by The Trustees of Indiana University, Bloomington, IN (US)
PCT Filed Apr. 11, 2019, PCT No. PCT/US2019/027082
§ 371(c)(1), (2) Date Oct. 9, 2020,
PCT Pub. No. WO2019/200166, PCT Pub. Date Oct. 17, 2019.
Claims priority of provisional application 62/656,306, filed on Apr. 11, 2018.
Prior Publication US 2021/0387175 A1, Dec. 16, 2021
Int. Cl. B01L 3/00 (2006.01); G01N 33/68 (2006.01)
CPC B01L 3/502 (2013.01) [G01N 33/6848 (2013.01); B01L 2200/025 (2013.01); B01L 2200/16 (2013.01); B01L 2300/0609 (2013.01); B01L 2300/069 (2013.01); B01L 2300/12 (2013.01); B01L 2400/027 (2013.01)] 20 Claims
OG exemplary drawing
 
1. A protein detection cartridge comprising:
a lid including an opening;
a base coupled to the lid and including a recess;
a column holder removably positioned in the opening, the column holder including an aperture;
an antibody column received within the aperture and moveable within the aperture;
a waste pad removably positioned in the recess of the base;
a spray tip holder positioned in the base, the spray tip holder including a recess; and
an enzyme immobilized membrane positioned in the recess of the spray tip holder;
wherein the column holder is movable between a first position where the antibody column is positioned over the waste pad and a second position where the antibody column is positioned over the enzyme immobilized membrane; and
wherein, with the column holder in the second position, the antibody column is configured to be pushed downwardly into the recess of the spray tip holder and into contact with the enzyme immobilized membrane.