	US 11,998,016 B2
	*Use of Chaetomium globosum* strain in controlling fusarium crown rot of wheat**
Fei Xu, Henan (CN); Yuli Song, Henan (CN); Junmei Wang, Henan (CN); Jiaojiao Zhang, Henan (CN); Zixing Han, Henan (CN); Yahong Li, Henan (CN); Ruijie Shi, Henan (CN); Lijuan Li, Henan (CN); and Lulu Liu, Henan (CN)
Assigned to Institute of Plant Protection, Henan Academy of Agricultural Sciences, Zhengzhou (CN)
Filed by Institute of Plant Protection, Henan Academy of Agricultural Sciences, Henan (CN)
Filed on Apr. 19, 2021, as Appl. No. 17/233,732.
Claims priority of application No. 202010832744.5 (CN), filed on Aug. 18, 2020.
Prior Publication US 2022/0053771 A1, Feb. 24, 2022
Int. Cl. A01N 63/30 (2020.01); A01N 25/04 (2006.01); C12N 15/00 (2006.01)

CPC A01N 63/30 (2020.01) [A01N 25/04 (2013.01); C12N 15/00 (2013.01)]

3 Claims

1. A method for controlling Fusarium crown rot (FCR) of wheat, the method comprising applying a Chaetomium globosum strain or a biocontrol agent containing the Chaetomium globosum strain to a FCR fungus,

wherein the Chaetomium globosum strain is strain 12XP1-2-3 deposited at China General Microbiological Culture Collection Center (CGMCC) on Jan. 23, 2019 with an accession number of CGMCC No. 17183;

wherein strain 12XP1-2-3 is identified by PCR using sequences ITS1 (5′-TCC GTA GGT GAA CCT GCG G-3′) and ITS4 (5′-TCC TCC GCT TAT TGA TAT GC-3′) as primers for amplification of DNA fragments containing ITS1, 5.8S rDNA and ITS2 in strain 12XP1-2-3; and

wherein wheat seeds are coated at a coating concentration of 5.0-5.3×10⁴ascospores per seed.