US 12,320,966 B2
Method and system for multi-view episcopic selective plane illumination microscope
Bin Yang, San Francisco, CA (US); and Loic Royer, San Francisco, CA (US)
Assigned to CZ Biohub SF, LLC, San Francisco, CA (US)
Filed by Chan Zuckerberg Biohub, Inc., San Francisco, CA (US)
Filed on Jan. 31, 2023, as Appl. No. 18/162,067.
Application 18/162,067 is a continuation of application No. PCT/US2021/044549, filed on Aug. 4, 2021.
Claims priority of provisional application 63/065,304, filed on Aug. 13, 2020.
Prior Publication US 2023/0168484 A1, Jun. 1, 2023
Int. Cl. H04N 13/207 (2018.01); G01N 21/64 (2006.01); G02B 21/00 (2006.01); G02B 26/10 (2006.01); H04N 13/282 (2018.01); H04N 23/55 (2023.01); H04N 23/56 (2023.01); H04N 23/74 (2023.01); H04N 13/156 (2018.01)
CPC G02B 21/008 (2013.01) [G01N 21/6456 (2013.01); G02B 21/0048 (2013.01); G02B 21/0076 (2013.01); G02B 26/101 (2013.01); H04N 13/207 (2018.05); H04N 13/282 (2018.05); H04N 23/55 (2023.01); H04N 23/56 (2023.01); H04N 23/74 (2023.01); G01N 2201/0636 (2013.01); G01N 2201/10 (2013.01); H04N 13/156 (2018.05)] 19 Claims
OG exemplary drawing
 
1. A multi-view selective plane imaging microscope comprising:
an illumination source;
an image detector;
a specimen stage operable to support a specimen having a first side and a second side opposing the first side, wherein an optical axis is disposed normal to the specimen;
a first objective disposed along the optical axis on the first side of the specimen;
a second objective disposed along the optical axis on the second side of the specimen;
an angle adjustment module operable to position a light sheet at a predetermined angle with respect to the optical axis;
a light sheet projection module operable to direct the light sheet to either the first objective or the second objective; and
an orientation control module operable to modify a scan direction of the light sheet.