US 12,320,817 B2
Enzyme-linked immunosorbent assay (ELISA) method and monoclonal antibody for giant panda relaxin-3 (RLN3)
Kailai Cai, Chengdu (CN); Rong Hou, Chengdu (CN); Yuliang Liu, Chengdu (CN); Jingchao Lan, Chengdu (CN); Mingxi Li, Chengdu (CN); Juan Wang, Chengdu (CN); Mengshi Zhang, Chengdu (CN); Shenfei Wang, Chengdu (CN); Feiping Li, Chengdu (CN); and Xianbiao Hu, Chengdu (CN)
Assigned to Chengdu Research Base of Giant Panda Breeding, Chengdu (CN)
Filed by Chengdu Research Base of Giant Panda Breeding, Chengdu (CN)
Filed on Aug. 19, 2024, as Appl. No. 18/808,126.
Claims priority of application No. 202311051419.5 (CN), filed on Aug. 21, 2023.
Prior Publication US 2025/0067756 A1, Feb. 27, 2025
Int. Cl. G01N 33/74 (2006.01); C07K 16/26 (2006.01); G01N 33/58 (2006.01)
CPC G01N 33/74 (2013.01) [C07K 16/26 (2013.01); G01N 33/58 (2013.01); G01N 2333/64 (2013.01)] 6 Claims
 
1. An enzyme-linked immunosorbent assay (ELISA) method for giant panda relaxin-3 (RLN3), comprising:
conducting ELISA with an anti-giant panda RLN3 monoclonal antibody as a coating antibody and an anti-RLN3 polyclonal antibody as a labeling antibody, wherein
the anti-giant panda RLN3 monoclonal antibody is secreted by a hybridoma cell line RLN-3 and the hybridoma cell line RLN-3 is deposited in China Center for Type Culture Collection (CCTCC) of Wuhan University, Wuhan, China on Jan. 7, 2021, with an accession number of CCTCC NO: C202129; and
the anti-giant panda RLN3 monoclonal antibody comprises a heavy-chain variable region with the amino acid sequence shown in SEQ ID NO: 1 and a light-chain variable region with the amino acid sequence shown in SEQ ID NO: 2.