US 12,320,815 B2
Compositions and methods for enrichment and detection of ubiquitin and ubiquitin conjugates
Mengwen Zhang, New Haven, CT (US); Jason Berk, Hamden, CT (US); and Mark Hochstrasser, Hamden, CT (US)
Assigned to Yale University, New Haven, CT (US)
Filed by YALE UNIVERSITY, New Haven, CT (US)
Filed on Oct. 1, 2020, as Appl. No. 17/061,250.
Claims priority of provisional application 62/909,545, filed on Oct. 2, 2019.
Prior Publication US 2021/0102950 A1, Apr. 8, 2021
Int. Cl. C07K 14/01 (2006.01); C07K 14/00 (2006.01); C07K 17/02 (2006.01); G01N 33/68 (2006.01)
CPC G01N 33/6842 (2013.01) [C07K 14/001 (2013.01); C07K 17/02 (2013.01); G01N 2440/36 (2013.01)] 18 Claims
 
1. An ubiquitin-binding peptide having the sequence of SEQ ID NO: 1 or SEQ ID NO: 5,
wherein Z is a sequence of amino acids or amino acid analogs having the sequence (Xaa)n or Z is L;
wherein n is 6;
wherein each Xaa is independently selected from the group consisting of alanine, arginine, asparagine, aspartic acid, cysteine, glutamine, glutamic acid, glycine, histidine, isoleucine, leucine, lysine, methionine, phenylalanine, proline, serine, threonine, tryptophan, tyrosine, valine, selenocysteine, penicillamine, homocysteine, and selenomethionine;
provided that at least one Xaa is selected from the group consisting of cysteine, selenocysteine, penicillamine, homocysteine, methionine, and selenomethionine;
wherein L is a chemical moiety selected from the group consisting of beta-alanine, 4-aminobutyric acid, 2-aminoethoxyl acetic acid, 5-aminovaleric acid, 6-aminohexanoic acid, 8-amino-3,6-dioxaoctanoic acid, 12-amino-4,7,10-trioxadodecanoic acid, 15-amino-4,7,10,13-tetraoxapenta-decanoic acid, trioxatridecan-succinamic acid, 11-azido-3,6,9-trioxaundecanoic acid, 2-[2-(2-propyn-1-yloxy)ethoxy]ethanoic acid, and 4,7,10,13,16-pentaoxanonadecanedioic acid di(N-succinimidyl)ester.