US 12,320,809 B2
Method for measuring viral antigen in sample, antibody set, and reagent kit
Keiko Higuchi, Kobe (JP); Naoki Nishiyama, Kobe (JP); Megumi Goto, Kobe (JP); and Nobuyuki Ide, Kobe (JP)
Assigned to SYSMEX CORPORATION, Kobe (JP)
Filed by SYSMEX CORPORATION, Kobe (JP)
Filed on Jul. 28, 2021, as Appl. No. 17/387,409.
Claims priority of application No. 2020-128480 (JP), filed on Jul. 29, 2020.
Prior Publication US 2022/0034883 A1, Feb. 3, 2022
Int. Cl. G01N 33/569 (2006.01); G01N 33/543 (2006.01); G01N 33/563 (2006.01)
CPC G01N 33/56983 (2013.01) [G01N 33/54326 (2013.01); G01N 33/563 (2013.01)] 18 Claims
 
1. A method for measuring a nucleoprotein of severe acute respiratory syndrome coronavirus (SARS-COV) or SARS-COV-2 in a sample by using a capture antibody and a detection antibody, the method comprising
forming a sandwich immune complex that contains the capture antibody, the nucleoprotein of SARS-COV or SARS-COV-2 and the detection antibody,
the capture antibody comprising a heavy chain variable region that comprises complementary determining region 1 (CDR1) comprising the amino acid sequence of SEQ ID NO: 1, CDR2 comprising the amino acid sequence of SEQ ID NO: 2 and CDR3 comprising the amino acid sequence of SEQ ID NO: 3, and a light chain variable region that comprises CDR1 comprising the amino acid sequence of SEQ ID NO: 4, CDR2 comprising the amino acid sequence of SEQ ID NO: 5 and CDR3 comprising the amino acid sequence of SEQ ID NO: 6, and
the detection antibody comprising a heavy chain variable region that comprises CDR1 comprising the amino acid sequence of SEQ ID NO: 7, CDR2 comprising the amino acid sequence of SEQ ID NO: 8 and CDR3 comprising the amino acid sequence of SEQ ID NO: 9, and a light chain variable region that comprises CDR1 comprising the amino acid sequence of SEQ ID NO: 10, CDR2 comprising the amino acid sequence of SEQ ID NO: 11 and CDR3 comprising the amino acid sequence of SEQ ID NO:12,
wherein the CDRs of the capture antibody and the detection antibody are determined based on the Kabat method.