US 12,320,753 B2
Methods for determining adjuvanted protein concentration and percent adsorption using intrinsic fluorescence
Salvador Fernando Ausar, Markham (CA); Cristopher Roque, Mono (CA); Kirsten April Strahlendorf, North York (CA); and Nausheen Rahman, North York (CA)
Assigned to SANOFI PASTEUR LIMITED, Toronto (CA)
Appl. No. 16/982,244
Filed by Sanofi Pasteur Limited, Toronto (CA)
PCT Filed Mar. 19, 2019, PCT No. PCT/IB2019/052232
§ 371(c)(1), (2) Date Sep. 18, 2020,
PCT Pub. No. WO2019/180618, PCT Pub. Date Sep. 26, 2019.
Claims priority of provisional application 62/645,367, filed on Mar. 20, 2018.
Prior Publication US 2021/0025827 A1, Jan. 28, 2021
Int. Cl. G01N 21/64 (2006.01); G01N 33/58 (2006.01)
CPC G01N 21/6486 (2013.01) [G01N 33/582 (2013.01)] 17 Claims
OG exemplary drawing
 
1. A method for determining the concentration of a selected compound in a composition comprising an adjuvanted complex of the selected compound, said method comprising:
(a) obtaining a fluorescence intensity value for a composition comprising an adjuvanted complex of a selected compound, wherein the selected compound includes at least one aromatic amino acid, and wherein said composition is excited at a wavelength of between 250 and 300 nm, and emission spectrum is determined at a wavelength of between 300 and 500 nm to obtain the fluorescence intensity value for the composition; and
(b) comparing the fluorescence intensity value obtained in (a) with a calibration curve prepared using fluorescence values of at least three calibration samples comprising different known concentrations of a representative compound that is representative of the selected compound to thereby determine the concentration of the selected compound in the composition comprising the adjuvanted complex of the selected compound assayed in (a).