US 12,320,752 B2
System level calibration
Karl Garsha, Sahuarita, AZ (US); and Dustin Harshman, Tucson, AZ (US)
Assigned to Ventana Medical Systems, Inc., Tucson, AZ (US)
Filed by Ventana Medical Systems, Inc., Tucson, AZ (US)
Filed on Aug. 4, 2023, as Appl. No. 18/365,805.
Application 18/365,805 is a continuation of application No. 16/680,319, filed on Nov. 11, 2019, granted, now 11,747,280.
Application 16/680,319 is a continuation of application No. PCT/EP2018/067095, filed on Jun. 26, 2018.
Claims priority of provisional application 62/525,818, filed on Jun. 28, 2017.
Prior Publication US 2023/0375477 A1, Nov. 23, 2023
This patent is subject to a terminal disclaimer.
Int. Cl. G01N 21/64 (2006.01); G01N 21/27 (2006.01); G02B 21/36 (2006.01); G06T 5/92 (2024.01); H04N 23/73 (2023.01)
CPC G01N 21/6458 (2013.01) [G01N 21/274 (2013.01); G01N 21/6428 (2013.01); G02B 21/365 (2013.01); G06T 5/92 (2024.01); H04N 23/73 (2023.01); G01N 2021/6439 (2013.01); G06T 2207/10056 (2013.01); G06T 2207/10064 (2013.01); G06T 2207/10144 (2013.01)] 20 Claims
OG exemplary drawing
 
1. A method of imaging a biological sample stained with one or more stains, each stain identifying a particular target within the biological sample, comprising:
setting a first power output of a light source for a first channel such that a first predetermined power value is reached;
adjusting a first exposure time of a camera to yield a first target intensity at the first predetermined power value for the first channel;
acquiring a first image of the biological sample in a first channel based on the adjusted first exposure time; and
adjusting pixel data in the first acquired image based on a first derived image correction factor specific to one of the one or more stains within the first channel, wherein the first derived image correction factor is computed by (i) normalizing brightness values for a plurality of stains; and (ii) subtracting the normalized brightness value for the first stain from the integer 1.