US 12,319,964 B2
Detection and digital quantitation of multiple targets
Eleen Yee Lam Shum, San Carlos, CA (US); Hei Mun Christina Fan, Palo Alto, CA (US); Stephen P.A. Fodor, Palo Alto, CA (US); Janice Hoiyi Lai, Mountain View, CA (US); Jung Won Keum, Palo Alto, CA (US); and Haeun Grace Lee, Palo Alto, CA (US)
Assigned to Countable Labs, Inc., Palo Alto, CA (US)
Filed by COUNTABLE LABS, INC., Palo Alto, CA (US)
Filed on Jun. 17, 2024, as Appl. No. 18/745,574.
Application 18/745,574 is a continuation of application No. 18/481,950, filed on Oct. 5, 2023, granted, now 12,049,668, issued on Jul. 30, 2024.
Application 18/481,950 is a continuation of application No. 18/085,217, filed on Dec. 20, 2022, granted, now 11,834,714, issued on Dec. 5, 2023.
Application 18/085,217 is a continuation of application No. PCT/US2022/053413, filed on Dec. 19, 2022.
Claims priority of provisional application 63/291,813, filed on Dec. 20, 2021.
Prior Publication US 2024/0409998 A1, Dec. 12, 2024
This patent is subject to a terminal disclaimer.
Int. Cl. C12Q 1/6876 (2018.01); G01N 21/64 (2006.01); G01N 33/542 (2006.01)
CPC C12Q 1/6876 (2013.01) [G01N 21/6428 (2013.01); G01N 33/542 (2013.01); C12Q 2600/156 (2013.01); C12Q 2600/16 (2013.01); G01N 2021/6439 (2013.01)] 20 Claims
 
1. A method comprising:
(a) providing a plurality of partitions comprising at least 500,000 partitions, wherein the plurality of partitions comprises:
(i) a plurality of target nucleic acid molecules; and
(ii) processing materials,
wherein a partition of the plurality of partitions comprises two or fewer target nucleic acid molecules of the plurality of target nucleic acid molecules;
(b) in the plurality of partitions, reacting the processing materials with the nucleic acid molecules;
(c) using an imaging system to scan the plurality of partitions across a set of channels to detect signals from at least a subset of the plurality of partitions, wherein each channel of the set of channels comprises a different filter configuration, wherein the number of targets of the set of targets is greater than the number of channels of the set of channels; and
(d) using the signals detected in (c) to identify at least a subset of the plurality of target nucleic acid molecules.