US 12,319,953 B2
Biologic machines for the detection of biomolecules
Jeff Albrecht, Los Angeles, CA (US); and Andrew J. Conrad, Malibu, CA (US)
Assigned to Laboratory Corporation of America Holdings, Burlington, NC (US)
Filed by Laboratory Corporation of America Holdings, Burlington, NC (US)
Filed on Sep. 30, 2022, as Appl. No. 17/937,061.
Application 16/857,353 is a division of application No. 13/772,514, filed on Feb. 21, 2013, granted, now 10,662,459, issued on May 26, 2020.
Application 17/937,061 is a continuation of application No. 16/857,353, filed on Apr. 24, 2020, granted, now 11,479,801.
Claims priority of provisional application 61/661,059, filed on Jun. 18, 2012.
Claims priority of provisional application 61/614,645, filed on Mar. 23, 2012.
Prior Publication US 2023/0021971 A1, Jan. 26, 2023
This patent is subject to a terminal disclaimer.
Int. Cl. C12Q 1/04 (2006.01); G01N 33/569 (2006.01)
CPC C12Q 1/04 (2013.01) [G01N 33/569 (2013.01); G01N 33/56983 (2013.01)] 17 Claims
 
1. A system for detection of a microorganism in a sample, comprising:
a lytic bacteriophage capable of infecting the microorganism to generate progeny bacteriophage and a soluble enzyme released when the microorganism is lysed by the progeny bacteriophage;
and a device comprising:
a first chamber configured for containing the sample and the lytic bacteriophage, wherein the first chamber comprises a port for adding the lytic bacteriophage, an inlet for introducing the sample, and one or more filters located between the port for adding lytic bacteriophage and the inlet for introducing the sample;
and a detection chamber comprising a detector configured to detect a detectable product of a reaction catalyzed by the released soluble enzyme found in solution in the detection chamber, wherein detection of the detectable product indicates that the microorganism is present in the sample.