US 12,319,951 B2
Enzymatically synthesized omega-3 structured phospholipids
Wilson Martinez Gutierrez, Barranquilla (CO); Alvaro Jose Garcia Padilla, Barranquilla (CO); Alfredo de Jesús Puche Simanca, Barranquilla (CO); and Tatiana Lucia Yepes Bustillo, Barranquilla (CO)
Assigned to C.I. Naturmega S.A., Barranquilla (CO)
Filed by Wilson Martinez Gutierrez, Barranquilla (CO); Alvaro Jose Garcia Padilla, Barranquilla (CO); Alfredo de Jesús Puche Simanca, Barranquilla (CO); and Tatiana Lucia Yepes Bustillo, Barranquilla (CO)
Filed on Sep. 22, 2022, as Appl. No. 17/951,048.
Claims priority of provisional application 63/246,898, filed on Sep. 22, 2021.
Prior Publication US 2023/0091294 A1, Mar. 23, 2023
This patent is subject to a terminal disclaimer.
Int. Cl. C12P 19/44 (2006.01); C12P 13/00 (2006.01)
CPC C12P 19/44 (2013.01) [C12P 13/001 (2013.01)] 14 Claims
 
1. A process for the incorporation of omega-3 and omega-6 fatty acids into polar lipid molecules present in phospholipids and glycolipids, which process comprises the steps of: (a) conducting an enzymatic exchange reaction between the fatty acids present in the polar lipids of the phospholipids and glycolipds and the omega-3 and omega-6 fatty acids, to obtain an oil with a high content of polar lipids and omega-3 fatty acids and omega-6 fatty acids, said enzymatic exchange reaction being conducted in the presence of a lipase or phospholipase enzyme selected from the group consisting of a chimera produced by the fusion of the genes of the lipase from Thermomyces lanuginosus and the phospholipase A1 from Fusarium oxysporum (Lecitase Ultra), Phospholipase C (Purifine), and Candida antarctica lipase B (CALB); (b) a stage of concentration of the polar lipid and omega-3 and omega-6 fatty acids content of the oil obtained in step (a) by supercritical fractionation or molecular distillation; (c) a new feeding stage of concentrated omega-3 oil or omega-6 to increase the incorporation of omega-3 and omega-6 in polar lipids and achieve a higher omega-3 and omega-6 content in the product; and (d) an ultrafiltration stage under a nitrogen atmosphere to give the desired consistency and appearance to the final oil.