US 12,319,948 B2
Pterin-dependent biocatalysts and uses thereof
Pamela Peralta-Yahya, Atlanta, GA (US); and Amy M. Ehrenworth, Atlanta, GA (US)
Assigned to GEORGIA TECH RESEARCH CORPORATION, Atlanta, GA (US)
Filed by Georgia Tech Research Corporation, Atlanta, GA (US)
Filed on Jul. 21, 2021, as Appl. No. 17/381,553.
Application 17/381,553 is a division of application No. 15/541,114, granted, now 11,085,060, previously published as PCT/US2015/068228, filed on Dec. 31, 2015.
Claims priority of provisional application 62/130,257, filed on Mar. 9, 2015.
Claims priority of provisional application 62/099,309, filed on Jan. 2, 2015.
Prior Publication US 2022/0090154 A1, Mar. 24, 2022
Int. Cl. C12P 17/18 (2006.01); C12N 9/02 (2006.01); C12N 9/04 (2006.01); C12N 9/78 (2006.01); C12N 9/88 (2006.01); C12N 15/52 (2006.01); C12P 7/00 (2006.01); C12P 7/22 (2006.01); C12P 13/00 (2006.01); C12P 13/22 (2006.01); C12P 17/10 (2006.01); C12P 17/12 (2006.01); C12P 17/16 (2006.01); C12P 19/60 (2006.01)
CPC C12P 17/182 (2013.01) [C12N 9/0006 (2013.01); C12N 9/0036 (2013.01); C12N 9/78 (2013.01); C12N 9/88 (2013.01); C12N 15/52 (2013.01); C12P 7/00 (2013.01); C12P 7/22 (2013.01); C12P 13/001 (2013.01); C12P 13/225 (2013.01); C12P 13/227 (2013.01); C12P 17/10 (2013.01); C12P 17/12 (2013.01); C12P 17/16 (2013.01); C12P 17/18 (2013.01); C12P 19/60 (2013.01); C12Y 101/01153 (2013.01); C12Y 106/99 (2013.01); C12Y 305/04016 (2013.01); C12Y 401/01028 (2013.01); C12Y 402/01096 (2013.01); C12Y 402/03012 (2013.01)] 14 Claims
 
1. A biocatalyst comprising:
a tetrahydrobiopterin source, wherein the tetrahydrobiopterin source is a tetrahydrobiopterin synthesis pathway comprising:
a GTP cyclohydrase, wherein the GTP cyclohydrolase is encoded by a nucleotide sequence comprising SEQ ID NO: 1, SEQ ID NO: 2, SEQ ID NO: 3, or SEQ ID NO: 4;
a pyruvoyl tetrahydropterin synthase, wherein the pyruvoyl tetrahydropterin synthase is encoded by a nucleotide sequence comprising SEQ ID NO: 5, SEQ ID NO: 6, SEQ ID NO: 7, or SEQ ID NO: 8; and
a sepiapterin reductase, wherein the sepiapterin reductase is encoded by a nucleotide sequence comprising SEQ ID NO: 9, SEQ ID NO: 10, or SEQ ID NO: 11; and
a pterin-dependent enzymatic pathway, wherein the pterin-dependent enzymatic pathway comprises tryptophan hydroxylase and aromatic-l-amino acid decarboxylase wherein the aromatic-1-amino acid decarboxylase is encoded by a nucleotide sequence comprising SEQ ID NO:17.