	US 12,319,945 B2
	Single step biocatalytic amidation
Peter Poechlauer, Linz (AT); Philipp Selig, Linz (AT); Christopher Zinganell, Linz (AT); Julia Pitzer, Graz (AT); Anton Glieder, Hofstatten an der Raab (AT); Wolfgang Kroutil, Graz (AT); and Kerstin Steiner, Graz (AT)
Assigned to PATHEON AUSTRIA GMBH & CO KG, Linz (AT)
Appl. No. 17/415,856
Filed by PATHEON AUSTRIA GMBH & CO KG, Linz (AT)
PCT Filed Dec. 3, 2019, PCT No. PCT/EP2019/083541 § 371(c)(1), (2) Date Jun. 18, 2021, PCT Pub. No. WO2020/126484, PCT Pub. Date Jun. 25, 2020.
Claims priority of application No. 18213434 (EP), filed on Dec. 18, 2018.
Prior Publication US 2022/0073958 A1, Mar. 10, 2022
Int. Cl. C12P 13/02 (2006.01); C12N 9/20 (2006.01)

CPC C12P 13/02 (2013.01) [C12Y 301/01003 (2013.01)]

8 Claims

1. A process for biocatalytic amidation of non-protected amino acids comprising the step of contacting a non-protected amino acid with an ammonia source in the presence of an organic solvent and a lipase enzyme, wherein the non-protected amino acid is L-proline, wherein the lipase is Candida antarctica lipase B (CalB) or a variant thereof, wherein the CalB variant has the amino acid substitutions T57A/A89T/G226R/R168K with reference to the numbering of SEQ ID NO: 1, and wherein the ammonia source is selected from the group consisting of ammonia, ammonium carbamate, ammonium carbonate, ammonium formate, and ammonium benzoate, and any mixtures thereof.