US 12,319,937 B2
Composition and method for culturing organoids
Jong-Man Yoo, Seongnam-si (KR); Soo-Jung Han, Seongnam-si (KR); and Myoung-Ok Nam, Daegu (KR)
Assigned to ORGANOIDSCIENCES, LTD., Gyeonggi-do (KR)
Appl. No. 16/618,268
Filed by ORGANOIDSCIENCES, LTD., Gyeonggi-do (KR)
PCT Filed May 29, 2018, PCT No. PCT/KR2018/006057
§ 371(c)(1), (2) Date Apr. 6, 2020,
PCT Pub. No. WO2018/221918, PCT Pub. Date Dec. 6, 2018.
Claims priority of application No. 10-2017-0065782 (KR), filed on May 29, 2017.
Prior Publication US 2020/0385683 A1, Dec. 10, 2020
Int. Cl. C12N 5/071 (2010.01); C07D 473/10 (2006.01)
CPC C12N 5/0679 (2013.01) [C07D 473/10 (2013.01); C12N 2501/999 (2013.01); C12N 2513/00 (2013.01)] 5 Claims
 
1. A method for forming a human small intestinal organoid comprising: culturing (i) a human intestinal tissue fragment or (ii) cells isolated from the human intestinal tissue fragment in a composition comprising a compound of the following chemical formula 1:

OG Complex Work Unit Chemistry
wherein a concentration of the compound in the composition is in the range of 5 μM to 100 μM.